Ouviña G B, Lemberg A, Bengochea L A
Cátedra de Fisiopatología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires.
Arch Int Physiol Biochim Biophys. 1993 Jan-Feb;101(1):77-8. doi: 10.3109/13813459308998133.
Liver microsomal glucuronidation of p-nitrophenol was measured in normal, 2 and 8 day bile duct ligated rats. At low aglycone concentration (0.24-0.8 mM) a Michaelis-Menten kinetic was registered in the three groups of animals but cholestatic rats showed a decrease of activity related to the time that cholestasis was maintained. At higher substrate concentration (1.2-2.0 mM), a very different behavior between the three groups was observed: normal rats showed a substrate inhibition phenomena; 2 day rats maintained plateau values and a remarkable activation effect of the activity was seen in the 8 day group. We concluded that cholestasis produced pronounced changes in p-nitrophenol glucuronidation pathway.
在正常大鼠、胆管结扎2天和8天的大鼠中测定了对硝基苯酚的肝脏微粒体葡萄糖醛酸化作用。在低苷元浓度（0.24 - 0.8 mM）时，三组动物均呈现米氏动力学，但胆汁淤积大鼠的活性随着胆汁淤积持续时间的延长而降低。在较高底物浓度（1.2 - 2.0 mM）时，三组之间观察到非常不同的行为：正常大鼠表现出底物抑制现象；2天大鼠保持平稳值，而在8天组中观察到活性有显著的激活作用。我们得出结论，胆汁淤积在对硝基苯酚葡萄糖醛酸化途径中产生了明显变化。
