Béress A, Wassermann O, Tahhan S, Bruhn T, Béress L, Kraiselburd E N, Gonzalez L V, de Motta G E, Chavez P I
Institute of Toxicology, Christian Albrechts-Universität, Kiel, Germany.
J Nat Prod. 1993 Apr;56(4):478-88. doi: 10.1021/np50094a005.
Anti-HIV-active polysaccharides and polyphenols were isolated from the brown seaweed Fucus vesiculosus by hot H2O extraction of both the intact and the homogenized algae. This was followed by XAD2 chromatography and by sequential precipitation of the non-adsorbed compounds with glacial HOAc and thereafter with EtOH. The precipitate was solubilized, dialyzed against distilled H2O, and chromatographed on SP-Sephadex C25 and on QAE-Sephadex A25. This was followed by gel filtration on Sephadex G50 and Sephadex G100 and finally by hplc on a Shodex Ionpak S-804 column. For comparison, the commercial product fucoidan, a sulfated algal polysaccharide, was also further purified by the chromatographic techniques mentioned above. The isolated freeze-dried fractions obtained by these procedures were tested for inhibition of both HIV-induced syncytium formation and HIV reverse transcriptase enzyme activity. Some of these fractions inhibited both of these activities at concentrations that were not cytotoxic.
通过对完整和匀浆的墨角藻进行热水提取，从褐藻墨角藻中分离出具有抗HIV活性的多糖和多酚。随后进行XAD2柱色谱分离，并用冰醋酸随后用乙醇对未吸附的化合物进行分步沉淀。将沉淀物溶解，用蒸馏水透析，并在SP-葡聚糖凝胶C25和QAE-葡聚糖凝胶A25上进行色谱分离。接着在葡聚糖凝胶G50和葡聚糖凝胶G100上进行凝胶过滤，最后在Shodex Ionpak S-804柱上进行高效液相色谱分析。作为对照，商业产品岩藻聚糖硫酸酯（一种硫酸化藻多糖）也通过上述色谱技术进一步纯化。通过这些程序获得的分离冻干级分进行了HIV诱导的合胞体形成抑制和HIV逆转录酶活性抑制测试。其中一些级分在无细胞毒性的浓度下抑制了这两种活性。
