Andeweg A C, Leeflang P, Osterhaus A D, Bosch M L
Laboratory of Immunobiology, National Institute of Public Health and Environmental Protection, Bilthoven, The Netherlands.
J Virol. 1993 Jun;67(6):3232-9. doi: 10.1128/JVI.67.6.3232-3239.1993.
To map the regions of the external envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) involved in the process of membrane fusion, we determined the syncytium-inducing capacity of a panel of transiently expressed chimeric envelope genes. This panel was generated by exchanging gene fragments between four previously studied envelope genes that exhibited a high degree of sequence homology yet displayed marked differences in syncytium-inducing capacity when expressed by recombinant vaccinia virus. The results demonstrate that multiple regions of the HIV-1 envelope glycoproteins are involved in syncytium formation. Some fragments, most notably those containing the V2 or V3 region, can transfer syncytium-inducing capacity to envelope proteins previously not capable of inducing syncytia. Moreover, it is shown that such regions functionally interact with other envelope regions, especially one encompassing the V4 and V5 regions of gp120 or a region encompassing part of gp41, to exert their function in membrane fusion.
为了绘制参与膜融合过程的1型人类免疫缺陷病毒（HIV-1）外膜糖蛋白区域图谱，我们测定了一组瞬时表达的嵌合包膜基因的诱导合胞体能力。该组基因是通过在四个先前研究的包膜基因之间交换基因片段产生的，这四个基因表现出高度的序列同源性，但当由重组痘苗病毒表达时，在诱导合胞体能力上表现出明显差异。结果表明，HIV-1包膜糖蛋白的多个区域参与合胞体形成。一些片段，最显著的是那些包含V2或V3区域的片段，可以将诱导合胞体的能力转移到以前不能诱导合胞体的包膜蛋白上。此外，研究表明，这些区域与其他包膜区域在功能上相互作用，特别是一个包含gp120的V4和V5区域或一个包含gp41部分的区域，以在膜融合中发挥其功能。
