细胞外病毒样颗粒中吉普赛（MDG4）逆转座子的存在。

Presence of the gypsy (MDG4) retrotransposon in extracellular virus-like particles.

作者信息

Syomin B V, Kandror K V, Semakin A B, Tsuprun V L, Stepanov A S

机构信息

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow.

出版信息

FEBS Lett. 1993 Jun 1;323(3):285-8. doi: 10.1016/0014-5793(93)81358-7.

DOI:10.1016/0014-5793(93)81358-7

PMID:7684710

Abstract

As a first step to investigate the functional activity of gypsy virus-like particles (VLPs) we explored the possibility of preservation of its VLP in extracellular form. The preparations containing extracellular gypsy VLP from Drosophila melanogaster and D. virilis were obtained. Full-length polyA+ RNA and polyA+ RNA-DNA complexes of gypsy were observed in both preparations. The polypeptides with some specificity to gypsy nucleic acids were identified in the obtained VLP preparations. These data accompanied by morphological characteristics of samples testify the presence of intact gypsy VLP in cultured media both from D. melanogaster and D. virilis cultivated cells.

摘要

作为研究吉普赛病毒样颗粒（VLPs）功能活性的第一步，我们探索了将其VLP以细胞外形式保存的可能性。获得了含有来自黑腹果蝇和 virilis果蝇细胞外吉普赛VLP的制剂。在两种制剂中均观察到了吉普赛的全长polyA+ RNA和polyA+ RNA-DNA复合物。在所获得的VLP制剂中鉴定出了对吉普赛核酸具有一定特异性的多肽。这些数据以及样品的形态特征证明，在来自黑腹果蝇和virilis果蝇培养细胞的培养基中存在完整的吉普赛VLP。