[吉普赛逆转座子（MDG4）细胞外病毒样颗粒中物质的一些生化特性]
[Some biochemical characteristics of substances from extracellular virus-like particles of the Gypsy retrotransposon (MDG4)].
作者信息
Semin B V, Kandror K V, Semakin A B, Tsuprun V L, Stepanov A S
出版信息
Biokhimiia. 1994 Apr;59(4):503-12.
To investigate the gypsy functional activity, the possibility of conservation of its genetic information in VLP in the extracellular form has been examined. Preparations containing extracellular gypsy VLP from D. melanogaster and D. virilis were obtained. Non degradative full-sized polyA+ RNA and polyA+ RNA-DNA complexes of gypsy were revealed in the both preparations. The polypeptides with some specificity to gypsy nucleic acids were identified in VLP preparations. Some morphological and physical characteristics of the VLP preparations were obtained. The data obtained suggest the presence of non-degradative gypsy VLP in cultured media of D. melanogaster and D. virilis cells.
为了研究吉卜赛病毒（gypsy）的功能活性，已对其遗传信息以细胞外形式保存在病毒样颗粒（VLP）中的可能性进行了研究。制备了含有来自黑腹果蝇（D. melanogaster）和粗壮果蝇（D. virilis）的细胞外吉卜赛病毒样颗粒的制剂。在这两种制剂中均发现了吉卜赛病毒的非降解全长多聚腺苷酸加尾RNA（polyA+ RNA）和多聚腺苷酸加尾RNA- DNA复合物。在病毒样颗粒制剂中鉴定出了对吉卜赛病毒核酸具有一定特异性的多肽。获得了病毒样颗粒制剂的一些形态和物理特征。所获得的数据表明，在黑腹果蝇和粗壮果蝇细胞的培养基中存在非降解的吉卜赛病毒样颗粒。
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