Berra E, Diaz-Meco M T, Dominguez I, Municio M M, Sanz L, Lozano J, Chapkin R S, Moscat J
Centro de Biología Molecular, Universidad Autonoma de Madrid, Canto Blanco, Spain.
Cell. 1993 Aug 13;74(3):555-63. doi: 10.1016/0092-8674(93)80056-k.
The requirement of protein kinase C zeta (zeta PKC) for maturation of X. laevis oocytes in response to insulin, p21ras, and phosphatidylcholine-hydrolyzing phospholipase C has recently been shown. Here we present experimental evidence demonstrating that activation of zeta PKC is not only necessary but also sufficient by itself to activate maturation in oocytes and to produce deregulation of growth control in mouse fibroblasts. Furthermore, by using a dominant kinase-defective mutant of zeta PKC, we confirm that this kinase is required for mitogenic activation in oocytes and fibroblasts. These results permit us to propose zeta PKC as a critical step downstream of p21ras in mitogenic signal transduction.
最近已表明，蛋白质激酶Cζ（ζ-PKC）对于非洲爪蟾卵母细胞响应胰岛素、p21ras和水解磷脂酰胆碱的磷脂酶C而成熟是必需的。在此，我们提供实验证据表明，ζ-PKC的激活不仅是卵母细胞激活成熟以及导致小鼠成纤维细胞生长控制失调所必需的，而且其自身就足以做到这一点。此外，通过使用ζ-PKC的显性激酶缺陷突变体，我们证实该激酶是卵母细胞和成纤维细胞有丝分裂原激活所必需的。这些结果使我们能够提出，ζ-PKC是有丝分裂原信号转导中p21ras下游的关键步骤。
