Coremans I E, Hagen E C, van der Voort E A, van der Woude F J, Daha M R, Breedveld F C
Department of Rheumatology, University Hospital, Leiden, The Netherlands.
Clin Exp Rheumatol. 1993 May-Jun;11(3):255-62.
In this study we determined the occurrence of anti-neutrophil cytoplasmic antibodies (ANCA) and the specificity of these antibodies (Ab) in the serum of patients with Felty's syndrome (FS) and in serum of patients with uncomplicated rheumatoid arthritis (RA). ANCA, detected by indirect immunofluorescence on ethanol-fixed neutrophils, was found in 77% of 30 patients with FS and in 36% of 50 RA patients. Ab against lactoferrin (LF) occurred more frequently in FS patients (50%) than in RA patients (4%), whereas reactivity against the other cytoplasmic antigens tested (proteinase-3, myeloperoxidase and elastase) did not differ significantly between these patient groups. The specific reactivity of FS sera with LF was established by demonstrating that the Ab reactivity resided in IgG, was located in the F(ab')2 fragments and could be inhibited from binding to the ELISA plate-bound LF by purified LF in the fluid phase. These results demonstrate that anti-LF Ab occur in FS and suggest that the detection of anti-LF Ab in patients with FS may be useful in the diagnosis of FS.
在本研究中，我们测定了费尔蒂综合征（FS）患者血清及无并发症类风湿关节炎（RA）患者血清中抗中性粒细胞胞浆抗体（ANCA）的发生率及其抗体（Ab）的特异性。通过对乙醇固定的中性粒细胞进行间接免疫荧光检测，在30例FS患者中有77%检测到ANCA，在50例RA患者中有36%检测到ANCA。抗乳铁蛋白（LF）抗体在FS患者（50%）中出现的频率高于RA患者（4%），而针对其他检测的胞浆抗原（蛋白酶-3、髓过氧化物酶和弹性蛋白酶）的反应性在这些患者组之间无显著差异。通过证明Ab反应性存在于IgG中、位于F(ab')2片段且可被液相中的纯化LF抑制与酶联免疫吸附测定（ELISA）板结合的LF结合，确定了FS血清与LF的特异性反应性。这些结果表明抗LF抗体在FS中出现，并提示在FS患者中检测抗LF抗体可能有助于FS的诊断。
