Ganguly B B
Biochemistry Unit, Indian Statistical Institute, Calcutta.
Mutat Res. 1993 Aug;295(3):135-48. doi: 10.1016/0921-8734(93)90015-u.
Spontaneous baseline frequencies of chromosome aberrations, micronucleus counts and cell division were analysed in peripheral lymphocytes of 127 normal healthy individuals in vitro. Cells were subjected to culture for 48 h in serum and PHA supplemented culture medium RPMI 1640. 100 metaphases were observed for chromosome aberrations and 1000 cells each for micronucleus counts and mitotic index. Regression analyses were carried out to see the effect of age on spontaneous abnormalities. The correlation of aberrations, micronucleus formation and mitotic index with donor's age is highly significant. The elevation of abnormalities and depression of mitotic index were linear to the increase of donor's age, with a higher frequency in males. Aged males and females from the age range of 40-70 years showed larger numbers of aberrations. Individuals with the smoking habit possessed higher frequencies of abnormalities than non-smokers.
对127名正常健康个体的外周血淋巴细胞进行体外培养，分析染色体畸变、微核计数和细胞分裂的自发基线频率。细胞在添加血清和PHA的RPMI 1640培养基中培养48小时。观察100个中期分裂相的染色体畸变情况，微核计数和有丝分裂指数各观察1000个细胞。进行回归分析以观察年龄对自发异常的影响。畸变、微核形成和有丝分裂指数与供体年龄的相关性非常显著。异常升高和有丝分裂指数降低与供体年龄增加呈线性关系，男性频率更高。年龄在40 - 70岁的老年男性和女性显示出更多的畸变。有吸烟习惯的个体比不吸烟者具有更高的异常频率。
