杆状巴尔通体甲硫氨酸起始tRNA基因的特征分析。

Characterization of the fMet initiator tRNA gene of Bartonella bacilliformis.

作者信息

Minnick M F

机构信息

Division of Biological Sciences, University of Montana, Missoula 59812.

出版信息

Gene. 1993 Sep 6;131(1):151-2. doi: 10.1016/0378-1119(93)90685-v.

DOI:10.1016/0378-1119(93)90685-v

PMID:7690338

Abstract

A ribosomal RNA operon was cloned from the agent of human Oroya fever, Bartonella bacilliformis. The 3' end of the operon contains a 77-nucleotide (nt) N-formylmethionine (fMet) initiator tRNA-encoding gene with 100% sequence identity to the fMet tRNA-encoding gene of Rhodobacter sphaeroides. The 3' region downstream from the gene has an 8-nt inverted repeat and 28 contiguous direct repeats of the heptamer 5'-TTCTCTA. To date, B. bacilliformis and R. sphaeroides are the only known eubacteria which have an fMet tRNA-encoding gene within the 3' end of their rRNA operons.

摘要

从人类奥罗亚热病原体巴氏杆菌中克隆出一个核糖体RNA操纵子。该操纵子的3'端包含一个77个核苷酸（nt）的N-甲酰甲硫氨酸（fMet）起始tRNA编码基因，与球形红杆菌的fMet tRNA编码基因具有100%的序列同一性。该基因下游的3'区域有一个8 nt的反向重复序列和28个连续的七聚体5'-TTCTCTA直接重复序列。迄今为止，巴氏杆菌和球形红杆菌是仅知的在其rRNA操纵子3'端含有fMet tRNA编码基因的真细菌。