HPRT-mutant frequency and lymphocyte characteristics of workers exposed to ionizing radiation on a sporadic basis: a comparison of two exposure indicators, job title and dose.

Using the clonal HPRT-mutant frequency assay, mutant frequencies of humans have been shown to rise following exposure to large doses of mutagens during radiotherapy, chemotherapy or after an atom bomb explosion. Success in relating mutant frequencies to exposure to high levels of mutagens has encouraged researchers to examine the effects of lower doses, such as those found among workers exposed at their jobs. In order to relate low doses of mutagens to biological effects, accurate characterization of exposure is critical, but most occupational studies are forced to use gross measures of exposure derived from job title or professional judgments as to potential exposure. Mutant frequencies and other relevant lymphocyte characteristics of 58 industrial workers were related to exposure status in two ways. When workers were classed as "exposed" or "unexposed" to ionizing radiation, no difference in any biological variable was seen between the two groups. When dosimeter readings were used as the exposure indicator, significant relationships appeared between dose and mutant frequency and CD4/CD8 lymphocyte subpopulation ratios. Mutant frequency was also positively related to age and smoking status. The time course of exposure and of appearance of mutant cells is discussed and it is suggested that this relationship receive attention in occupational studies of genotoxic effects.