Burrage T G, Trevejo R, Stone-Marschat M, Laegreid W W
Plum Island Animal Disease Center, United States Department of Agriculture, Greenport, New York 11944.
Virology. 1993 Oct;196(2):799-803. doi: 10.1006/viro.1993.1537.
A panel of monoclonal antibodies (MAbs) was generated against African horsesickness virus serotype 4 (AHSV/4). Three of the MAbs (SA6, OH3, and ME11) strongly neutralized the homologous virus and a heterologous type 4 isolate. The MAbs did not cross-neutralize AHS serotypes 1-3 or 5-9. The MAbs immunoprecipitated a viral protein of 108 kDa which co-migrated with VP2. Pretreatment with SA6 prevented mortality of 71% of day-old mice after intracranial injection of 100 LD50 of AHSV/4, while OH3 and ME11 significantly increased the average survival time of challenged animals. This study demonstrates that neutralizing epitope(s) for AHS are located on VP2 and that antibodies to these epitope(s) are protective in a neonatal mouse model.
制备了一组针对非洲马瘟病毒血清型4（AHSV/4）的单克隆抗体（MAb）。其中三种单克隆抗体（SA6、OH3和ME11）能强烈中和同源病毒及一种异源4型分离株。这些单克隆抗体不能交叉中和AHS血清型1 - 3或5 - 9。这些单克隆抗体免疫沉淀出一种与VP2共迁移的108 kDa病毒蛋白。用SA6预处理可使100 LD50的AHSV/4颅内注射后71%的新生小鼠免于死亡，而OH3和ME11显著延长了受攻击动物的平均存活时间。本研究表明，AHS的中和表位位于VP2上，针对这些表位的抗体在新生小鼠模型中具有保护作用。
