Fujino A
Department of Urology, Kitasato University School of Medicine.
Kaku Igaku. 1993 Jul;30(7):795-8.
The biodistribution of 125I labeled monoclonal antibody against gamma seminoprotein (gamma-Sm) in the nude mice bearing human benign prostatic hyperplasia xenograft was evaluated by whole body autoradiography and by counting of radioactivity in organs. The monoclonal antibody (murine Ig G1, K) to gamma-Sm which was established in this institute and its F(ab')2 fragment were radioiodinated using Iodogen method. The autoradiograms demonstrated specific uptake of 125I-intact Ig G as well as 125I-F(ab')2 within the prostatic adenoma xenografts 4 days after intravenous administrations. Radioactivity in the xenograft was relatively higher than those in the liver, kidney or lung. These results suggest that radio-labeled monoclonal antibody against gamma-Sm might be applicable for radioimmunodetection of prostatic tumors which produce gamma-Sm.
通过全身放射自显影和器官放射性计数，评估了125I标记的抗γ-精蛋白（γ-Sm）单克隆抗体在携带人良性前列腺增生异种移植物的裸鼠体内的生物分布。使用碘原法对本研究所制备的抗γ-Sm单克隆抗体（鼠Ig G1，κ）及其F(ab')2片段进行放射性碘化。放射自显影片显示，静脉注射后4天，前列腺腺瘤异种移植物内125I完整Ig G和125I-F(ab')2均有特异性摄取。异种移植物中的放射性相对高于肝脏、肾脏或肺部。这些结果表明，放射性标记的抗γ-Sm单克隆抗体可能适用于对产生γ-Sm的前列腺肿瘤进行放射免疫检测。
