Kuzu I, Bicknell R, Fletcher C D, Gatter K C
Nuffield Department of Pathology, John Radcliffe Hospital, Headington, Oxford.
Lab Invest. 1993 Sep;69(3):322-8.
Endothelial cells are important in initiating adhesive processes between circulating cells and extracellular structures and changes in their distribution are believed to be important in many pathologic conditions. Since little is known about the detailed distribution of adhesion molecules in human endothelium in different sites and circumstances, the present study has undertaken a detailed analysis of 5 of the putative most important adhesion molecules on a wide range of normal tissue endothelium. We have compared this reactivity with that seen in a comprehensive range of vascular tumors both benign and malignant.
Fresh samples of a wide range of normal tissues and vascular tumors were stained by antibodies against the following adhesion molecules; intercellular adhesion molecule-1 (CD54), E-selectin (endothelial cell adhesion molecule-1), vascular cell adhesion molecule-MUC-1, P-selectin (platelet activation-dependent granule to external membrane protein, CD62) and MUC-18 using either the APAAP immuno-alkaline phosphatase or an immunoperoxidase method.
Labeling of the endothelial cells in different normal tissues with intercellular adhesion molecule-1, P-selectin, and MUC-18 was heterogeneous both in terms of vessel size and strength of staining. Vascular cell adhesion molecule-1 and E-selectin were largely absent. The vascular tumors were likewise variable in their staining patterns which frequently differed from the immunophenotype of the reactive vessels surrounding the tumor.
This study demonstrates that the expression of adhesion molecules of the immunoglobulin and selectin family on normal tissue endothelium, and vascular tumors is much less predictable than that obtained with other vascular markers such as F8 RA, CD31, CD34, and CD36. Adhesion molecules show considerable heterogeneity of expression on vascular endothelium which presumably reflects their varied functions on different types of vessel. In general their expression is markedly reduced on vascular tumors.
内皮细胞在启动循环细胞与细胞外结构之间的黏附过程中起重要作用，其分布变化在许多病理状况中被认为至关重要。由于对不同部位和情况下人类内皮中黏附分子的详细分布了解甚少，本研究对一系列正常组织内皮上5种假定最重要的黏附分子进行了详细分析。我们将这种反应性与一系列良性和恶性血管肿瘤中的反应性进行了比较。
使用抗碱性磷酸酶抗过氧化物酶（APAAP）免疫碱性磷酸酶或免疫过氧化物酶方法，用针对以下黏附分子的抗体对一系列正常组织和血管肿瘤的新鲜样本进行染色：细胞间黏附分子-1（CD54）、E-选择素（内皮细胞黏附分子-1）、血管细胞黏附分子-MUC-1、P-选择素（血小板活化依赖性颗粒外膜蛋白，CD62）和MUC-18。
不同正常组织中的内皮细胞用细胞间黏附分子-1、P-选择素和MUC-18标记时，在血管大小和染色强度方面均存在异质性。血管细胞黏附分子-1和E-选择素基本未表达。血管肿瘤的染色模式同样多变，且常常不同于肿瘤周围反应性血管的免疫表型。
本研究表明，免疫球蛋白和选择素家族黏附分子在正常组织内皮和血管肿瘤上的表达比用其他血管标记物（如F8 RA、CD31、CD34和CD36）获得的表达更难预测。黏附分子在血管内皮上表现出相当大的表达异质性，这大概反映了它们在不同类型血管上的多种功能。一般来说，它们在血管肿瘤上的表达明显降低。
