Bose S, Ghosh P, Ghosh S, Chaudhury S, Bhattacharya S
Department of Zoology, Visva-Bharati University, Santiniketain, India.
Biomed Environ Sci. 1993 Jun;6(2):195-206.
Cytotoxicity of inorganic mercury to the liver of two species, Anabas testudineus and Sprague Dawley male rat was evaluated. Distribution kinetics of this metal in the different hepatic subcellular fractions were followed for 48 h after a single injection of [203Hg] mercuric nitrate at a dose of 4mg/kg b.w. Interaction of this metal with protein, DNA and RNA was also studied. In rat, nuclear and lysosomal fractions showed a significant increase in the protein content, while in fish, the amount of protein increased in all fractions except microsome. Comparatively more mercury was bound to protein in fish during the later phase of treatment. Retention of mercury in nuclear DNA of rat gradually declined from 15 min to 48 h of treatment, while, mitochondrial DNA binding to mercury increased from 15 min to 2 h of post injection and then declined in the later phase of the experiment. Such a biphasic binding pattern of mercury was shown by both the nuclear and mitochondrial DNA of the fish. The nuclear RNA of rat and mitochondrial and cytosolic RNA of both test species also showed a biphasic pattern of mercury binding, however, with a higher rate of binding in fish at the later phase of the experiment. The present study thus highlights that (a) mercury follows a definite distribution pattern in the subcellular fractions of the liver in both animal species, (b) cytosol is the major site of mercury accumulation.
评估了无机汞对两种生物（龟壳攀鲈和斯普拉格-道利雄性大鼠）肝脏的细胞毒性。在以4mg/kg体重的剂量单次注射[203Hg]硝酸汞后，跟踪该金属在不同肝脏亚细胞组分中的分布动力学48小时。还研究了该金属与蛋白质、DNA和RNA的相互作用。在大鼠中，核和溶酶体组分中的蛋白质含量显著增加，而在鱼类中，除微粒体外的所有组分中的蛋白质含量均增加。在处理后期，鱼类中与蛋白质结合的汞相对较多。在大鼠的核DNA中，汞的保留量在处理的15分钟至48小时逐渐下降，而线粒体DNA与汞的结合在注射后15分钟至2小时增加，然后在实验后期下降。鱼类的核DNA和线粒体DNA均呈现出这种汞的双相结合模式。大鼠的核RNA以及两种受试生物的线粒体和胞质RNA也呈现出汞结合的双相模式，然而，在实验后期，鱼类的结合率更高。因此，本研究强调：（a）汞在两种动物物种肝脏的亚细胞组分中遵循明确的分布模式，（b）胞质溶胶是汞积累的主要部位。
