Bose S, Ghosh P, Ghosh S, Bhattacharya S
Department of Zoology, Visva-Bharati University, Santiniketan, India.
Sci Total Environ. 1993;Suppl Pt 1:533-8. doi: 10.1016/s0048-9697(05)80055-5.
The present study tries to find out the kinetics of distribution of mercury in the different subcellular fractions of the liver in a freshwater perch Anabas testudineus over a period of 48 h after a single i.m. injection of [203Hg]mercuric nitrate at a dose of 4 mg/kg body weight. The fish were killed at 15 min, 2 h, 6 h and 48 h post injection. In addition the interaction of this metal with different biomolecules, viz., protein, DNA and RNA, was also investigated. Cytosol was found to be the major site of mercury accumulation. Moderate amounts of accumulation occurred in the nuclear, mitochondrial and microsomal fractions, although varying with time, while the lysosomal fraction did not reveal any spectacular retention of mercury. A significant increase in the protein content of nuclear, mitochondrial, lysosomal and cytosolic fractions was also noticed at different time periods of mercury injection. In the nuclear, microsomal and cytosolic proteins, mercury binding increased more significantly over time than the mitochondrial and lysosomal proteins. A biphasic binding pattern of mercury was seen in nuclear and mitochondrial DNA and mitochondrial and cytosolic RNA.
本研究试图探究淡水鲈龟壳攀鲈在单次肌肉注射4mg/kg体重的[203Hg]硝酸汞后48小时内，汞在肝脏不同亚细胞组分中的分布动力学。在注射后15分钟、2小时、6小时和48小时处死鱼。此外，还研究了这种金属与不同生物分子（即蛋白质、DNA和RNA）的相互作用。发现胞质溶胶是汞积累的主要部位。核、线粒体和微粒体组分中发生了适量的积累，尽管随时间变化，而溶酶体组分未显示出任何显著的汞保留。在汞注射的不同时间段，还注意到核、线粒体、溶酶体和胞质溶胶组分的蛋白质含量显著增加。在核、微粒体和胞质溶胶蛋白质中，汞结合随时间的增加比线粒体和溶酶体蛋白质更显著。在核和线粒体DNA以及线粒体和胞质溶胶RNA中观察到汞的双相结合模式。
