Re M C, Zauli G, Gibellini D, Furlini G, Ramazzotti E, Monari P, Ranieri S, Capitani S, La Placa M
Institute of Microbiology, University of Bologna, Italy.
AIDS. 1993 Aug;7(8):1049-55. doi: 10.1097/00002030-199308000-00004.
To determine the mechanism underlying the poor growth in vitro of haematopoietic progenitor cells isolated from HIV-1-infected patients.
Apoptotic death in liquid culture of bone-marrow CD34+ cells obtained from 11 HIV-1-seropositive patients and 18 HIV-1-seronegative donors was quantitatively monitored by a flow cytometry procedure.
No significant differences in the percentage of apoptotic cells were noted between the two groups immediately after purification. When CD34+ cells were placed in liquid cultures supplemented with 2 ng/ml interleukin-3, the number of apoptotic cells progressively and significantly (P < 0.05) increased in all HIV-1-seropositive patients, while it remained constant in HIV-1-seronegative individuals. Although all HIV-1-seropositive patients showed signs of active viral replication in the bone-marrow micro-environment, progenitor CD34+ cells did not show the presence of active and/or latent HIV-1 infection.
Our data demonstrate that CD34+ cells isolated from AIDS patients with active HIV-1 replication in bone-marrow accessory cells are committed to apoptotic death without being directly affected by productive infection.
确定从感染HIV-1的患者中分离出的造血祖细胞体外生长不良的潜在机制。
通过流式细胞术对11名HIV-1血清阳性患者和18名HIV-1血清阴性供者的骨髓CD34+细胞在液体培养中的凋亡死亡进行定量监测。
纯化后两组间凋亡细胞百分比无显著差异。当将CD34+细胞置于添加2 ng/ml白细胞介素-3的液体培养中时，所有HIV-1血清阳性患者的凋亡细胞数量逐渐且显著增加（P < 0.05），而在HIV-1血清阴性个体中保持恒定。尽管所有HIV-1血清阳性患者在骨髓微环境中均显示出活跃的病毒复制迹象，但祖细胞CD34+细胞未显示出活跃和/或潜伏的HIV-1感染。
我们的数据表明，从骨髓辅助细胞中存在活跃HIV-1复制的艾滋病患者中分离出的CD34+细胞会发生凋亡死亡，而未受到增殖性感染的直接影响。
