Wehle K, Küpper T, Marzahn S, Pfitzer P
Department of Cytopathology, Heinrich Heine University, Düsseldorf, Germany.
Cytopathology. 1993;4(4):225-9. doi: 10.1111/j.1365-2303.1993.tb00092.x.
Examination of Papanicolaou-stained bronchoalveolar lavage samples from cases with Pneumocystis carinii pneumonitis under ultra-violet light reveals alveolar macrophages packed with fluorescent inclusions. Immunoenzymatic staining of the alveolar macrophages with a monoclonal antibody specific for P. carinii (3F6) showed that these inclusions contain intact pneumocysts or their degradation products. Fluorescence microscopy of Papanicolaou-stained smears is advocated as a sensitive and specific method of diagnosing P. carinii infection.
在紫外线灯下检查卡氏肺孢子虫肺炎病例的巴氏染色支气管肺泡灌洗样本，发现肺泡巨噬细胞内充满荧光包涵体。用针对卡氏肺孢子虫的单克隆抗体（3F6）对肺泡巨噬细胞进行免疫酶染色显示，这些包涵体含有完整的肺孢子虫或其降解产物。巴氏染色涂片的荧光显微镜检查被认为是诊断卡氏肺孢子虫感染的一种灵敏且特异的方法。
