Maréchal-Drouard L, Khamiss O, Dietrich A
Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, Strasbourg, France.
Plant Mol Biol. 1993 Sep;22(6):1157-61. doi: 10.1007/BF00028985.
The initiator methionine transfer RNA (tRNA(fMet)) gene was identified on a 347 bp Eco RI-Hind III DNA fragment of the potato mitochondrial (mt) genome. The sequence of this gene shows 1 to 7 nucleotide differences with the other plant mt tRNAs(fMet) or tRNA(fMet) genes studied so far. Whereas the tRNA(fMet) gene is present as a single copy in the potato mt genome, a tRNA 'pseudogene' corresponding to 60% of a complete tRNA (from the 5' end to the variable region) and located at 105 nucleotides upstream of the tRNA(fMet) gene on the opposite strand was shown to be repeated at least three times. Furthermore, the physical environment of the tRNA(fMet) gene in the mt genome is very different among plants, which suggests that the tRNA(fMet) gene region has often been implicated in recombination events of plant mt genomes leading to important rearrangements in gene order.
起始甲硫氨酸转运RNA（tRNA(fMet)）基因是在马铃薯线粒体（mt）基因组的一个347 bp的Eco RI - Hind III DNA片段上鉴定出来的。该基因的序列与迄今为止研究的其他植物线粒体tRNA(fMet)或tRNA(fMet)基因相比，有1至7个核苷酸差异。虽然tRNA(fMet)基因在马铃薯线粒体基因组中以单拷贝形式存在，但在相反链上位于tRNA(fMet)基因上游105个核苷酸处，有一个对应于完整tRNA 60%（从5'端到可变区）的tRNA“假基因”，且该假基因至少重复了三次。此外，线粒体基因组中tRNA(fMet)基因的物理环境在不同植物间差异很大，这表明tRNA(fMet)基因区域常参与植物线粒体基因组的重组事件，导致基因顺序发生重要重排。
