Shimosegawa T, Abe T, Satoh A, Abe R, Kikuchi Y, Koizumi M, Toyota T
Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.
Gastroenterology. 1993 Oct;105(4):999-1008. doi: 10.1016/0016-5085(93)90942-6.
To provide a morphological basis for understanding the role of nitric oxide in the pancreas, the present study was designed to clarify the localization and distribution of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) activity, a marker of NO synthase, in the pancreas of several mammalian species, including humans.
NADPH-d activity was examined in the rat, guinea pig, dog, and human pancreas by histochemistry. In addition, the possibility of coproduction of NO and vasoactive intestinal polypeptide (VIP) was investigated by a combined use of histochemistry and immunohistochemistry.
In the pancreas, NADPH-d activity was localized in nerve fibers, nerve cell bodies, and the vascular endothelium. Nerve fibers with the enzyme activity were chiefly distributed in the exocrine pancreas and showed species differences in the distribution. Nerve fibers stained for NADPH-d were also observed in the endocrine pancreas, but the enzyme activity was not detected in the islet cells. Part of the nerve fibers and nerve cell bodies coexpressed NADPH-d activity and VIP-immunoreactivity.
These results suggest that NO may act as a neuronal mediator and an endothelium-derived relaxing factor and may physiologically interact with VIP in the mammalian pancreas.
为了提供形态学依据以理解一氧化氮在胰腺中的作用，本研究旨在阐明烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）活性（一氧化氮合酶的一种标志物）在包括人类在内的几种哺乳动物胰腺中的定位和分布。
采用组织化学方法检测大鼠、豚鼠、狗和人类胰腺中的NADPH-d活性。此外，通过联合使用组织化学和免疫组织化学方法研究一氧化氮和血管活性肠肽（VIP）共同产生的可能性。
在胰腺中，NADPH-d活性定位于神经纤维、神经细胞体和血管内皮。具有该酶活性的神经纤维主要分布在外分泌胰腺中，且在分布上存在种属差异。在内分泌胰腺中也观察到了NADPH-d染色的神经纤维，但在胰岛细胞中未检测到该酶活性。部分神经纤维和神经细胞体共表达NADPH-d活性和VIP免疫反应性。
这些结果表明，一氧化氮可能作为一种神经介质和内皮源性舒张因子，并可能在哺乳动物胰腺中与VIP发生生理相互作用。
