Vollmer G, Lightner V A, Carter C A, Siegal G P, Erickson H P, Knuppen R, Kaufman D G
Institut für Biochemische Endokrinologie, Medizinische Universität, Lübeck, FRG.
Pathobiology. 1993;61(2):67-76. doi: 10.1159/000163763.
Normal mesenchymal cells within developing embryonic organs and transformed stromal cells in organs undergoing spontaneous carcinogenesis have the capacity for normal or altered expression of the extracellular matrix glycoprotein tenascin (Tn). Mesenchymal cell constituents of normal adult organs show only a very limited tendency to deposit Tn in their extracellular matrix. In the present study, we investigated whether malignant human mesenchymal cells derived from uterine sarcomas or normal human endometrial stromal cells partially transformed via transfection with selected oncogenes have the capacity to produce and deposit Tn. We reached the following conclusions: (1) compared with normal endometrial tissues, uterine sarcomas show heterogeneous, but increased, immunoreactive staining patterns exclusively within the extracellular compartment, regardless of the histologic subtype of the tumor; (2) in vitro, all normal and transfected stromal cells and cell lines examined secreted Tn into the tissue culture medium; (3) this secretory ability was not translated into morphologic uniformity, since immunoreactivity detected by confocal laser scanning microscopy was observed in only selected cell populations; (4) also, the deposition and the incorporation of Tn depended upon the density of transfected cells, and (5) double-staining experiments revealed that Tn could always be localized in close proximity to fibronectin. In summary, the production of Tn is increased in most cases of human uterine sarcoma. The capacity of stromal cells to deposit Tn in a matrix-like structure in vitro, rather than increase production of Tn, is correlated with the degree of neoplastic progression.
发育中的胚胎器官内的正常间充质细胞以及正在经历自发癌变的器官中的转化基质细胞，具有正常或改变表达细胞外基质糖蛋白腱生蛋白（Tn）的能力。正常成体器官的间充质细胞成分在其细胞外基质中沉积Tn的倾向非常有限。在本研究中，我们调查了源自子宫肉瘤的恶性人间充质细胞或通过用选定的癌基因转染而部分转化的正常人子宫内膜基质细胞是否有能力产生和沉积Tn。我们得出了以下结论：（1）与正常子宫内膜组织相比，子宫肉瘤显示出异质性但增加的免疫反应性染色模式，仅在细胞外区域，无论肿瘤的组织学亚型如何；（2）在体外，所有检测的正常和转染的基质细胞及细胞系都将Tn分泌到组织培养基中；（3）这种分泌能力并未转化为形态学上的一致性，因为共聚焦激光扫描显微镜检测到的免疫反应性仅在选定的细胞群体中观察到；（4）此外，Tn的沉积和掺入取决于转染细胞的密度，并且（5）双重染色实验表明Tn总是可以定位在纤连蛋白附近。总之，在大多数人类子宫肉瘤病例中，Tn的产生增加。基质细胞在体外以基质样结构沉积Tn的能力，而非增加Tn的产生，与肿瘤进展程度相关。
