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用于测定红细胞抗原和抗体的(部分)自动化凝胶技术

[(Partially) automated gel techniques for determining erythrocyte antigens and antibodies].

作者信息

Kreutzig B, Weisshaar D

机构信息

Blutspendedienst Hessen des DRK, Institut Kassel, BRD.

出版信息

Beitr Infusionsther. 1993;31:152-6.

PMID:7693245
Abstract

Today, in addition to the classical test tube technology to determine red blood cell antigens and antibodies, not only the microtitration plate is increasingly used, but also the gel card. Since this new technology is easy to practice and since it further provides a higher specificity and sensitivity, we investigated whether, and if so to what extent, the gel card can be automated. On the basis of our own experience spanning many years, we were able to show that antigen determinations and antibody screening can be automated. Once the samples to be tested and the tests to be performed have been recorded by electronic data processing and combined in a job list, any sampler can distribute liquids, such as reagents and serum and/or plasma as well as red blood cell suspensions, on the basis of these data. A positive sample identification is made feasible by bar coding sample tubes and gel cards; the pipetting action is organized via the bar codes. Incubation and centrifugation are of secondary importance for automation. At this time, an automatic evaluation of gel cards is not yet possible so that result interpretation must be visual and the input of results must be manual. For documentation, however, laboratory books and result interpretation reports can be printed out automatically.

摘要

如今,除了用于确定红细胞抗原和抗体的经典试管技术外,微量滴定板不仅越来越多地被使用,凝胶卡也被广泛应用。由于这项新技术易于操作,并且具有更高的特异性和灵敏度,我们研究了凝胶卡是否能够实现自动化,如果可以,能达到何种程度。基于我们多年的经验,我们能够证明抗原测定和抗体筛查可以实现自动化。一旦通过电子数据处理记录了待检测的样本和要进行的检测,并将它们组合成一个任务列表,任何采样器都可以根据这些数据分配液体,如试剂、血清和/或血浆以及红细胞悬液。通过对样本管和凝胶卡进行条形码编码,可以实现对样本的准确识别;加样操作通过条形码进行组织。孵育和离心对于自动化来说并不重要。目前,凝胶卡还无法自动评估,因此结果解读必须通过肉眼进行,结果输入也必须手动完成。然而,对于文档记录,实验室手册和结果解读报告可以自动打印出来。

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