Scala C, Preda P, Cenacchi G, Martinelli G N, Manara G C, Pasquinelli G
Istituto di Microscopia Elettronica Clinica, Università di Bologna, Italy.
Histochem J. 1993 Sep;25(9):670-7. doi: 10.1007/BF00157881.
We describe a new polychrome stain and simultaneous methods of histological, histochemical and immunocytochemical staining performed on sections from human tissues embedded in the new hydrophilic resin Bioacryl. The polychrome stain involves the sequential use of Harris' Haematoxylin, silver methenamine, Light Green and Eosin or Safranin dyes and provides a highly specific visualization of the overall cytological tissue architecture. When histochemical, immunocytochemical, and polychrome stains are performed together on the same section, crisp images are obtained, yielding simultaneous data of histochemical and immunological reactivities with clear tissue architecture.
我们描述了一种新的多色染色法以及在包埋于新型亲水性树脂Bioacryl中的人体组织切片上进行组织学、组织化学和免疫细胞化学染色的同步方法。这种多色染色法依次使用哈里斯苏木精、亚甲胺银、亮绿和伊红或番红染料，能高度特异性地显示整个细胞组织结构。当在同一切片上同时进行组织化学、免疫细胞化学和多色染色时，可获得清晰的图像，同时得到具有清晰组织结构的组织化学和免疫反应性数据。
