Radulovic S, Speed R, Feng H M, Taylor C, Walker D H
Dept. of Pathology, University of Texas Medical Branch, Galveston 77555-0609.
J Infect Dis. 1993 Nov;168(5):1292-5. doi: 10.1093/infdis/168.5.1292.
All currently available assays for antibodies to the etiologic agents of spotted fever group rickettsioses detect reactivity with antigens of lipopolysaccharides and the major cell wall proteins, which contain epitopes that are shared among many spotted fever group rickettsiae. The hypothesis of this study was that a monoclonal antibody to a species-specific epitope of Rickettsia rickettsii would be blocked from binding to the rickettsial surface if the rickettsiae were incubated previously with serum containing species-specific antibodies to the epitope. In an EIA, binding of monoclonal antibody 5C10-F3 to R. rickettsii was blocked by convalescent sera from patients with Rocky Mountain spotted fever but not from those with Mediterranean spotted fever, endemic typhus, or Q fever or normal subjects. This assay should be useful in determining the origin of the high seroprevalence of spotted fever rickettsial antibodies in certain geographic regions and establishing a species-specific diagnosis in patients with undetermined rickettsial exposure.
目前所有用于检测斑点热群立克次体病病原体抗体的检测方法，均能检测到与脂多糖抗原及主要细胞壁蛋白的反应性，这些蛋白包含许多斑点热群立克次体共有的表位。本研究的假设是，如果立克次体事先与含有针对该表位的种特异性抗体的血清一起孵育，那么针对立氏立克次体种特异性表位的单克隆抗体将被阻止与立克次体表面结合。在酶免疫分析中，单克隆抗体5C10-F3与立氏立克次体的结合被落基山斑点热患者的恢复期血清所阻断，但未被地中海斑点热、地方性斑疹伤寒、Q热患者或正常受试者的血清阻断。该检测方法在确定某些地理区域斑点热立克次体抗体高血清阳性率的来源以及对立克次体暴露情况未明的患者进行种特异性诊断方面应会有用。
