Ceccatelli S, Lundberg J M, Zhang X, Aman K, Hökfelt T
Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Brain Res. 1994 Sep 12;656(2):381-95. doi: 10.1016/0006-8993(94)91483-4.
In the present immunohistochemical study the distribution of nitric oxide synthase (NOS) was studied in various autonomic ganglia and in related peripheral tissues of the rat. For comparison some other neuronal markers including acetylcholinesterase and tyrosine hydroxylase as well as several neuropeptides were analysed on adjacent or the same sections. The distribution of NOS-like immunoreactivity (LI) and of these other markers has been semiquantitatively summarized. In some parasympathetic ganglia such as the sphenopalatine and submandibular ganglia NOS-LI was present in most ganglion cells, at least partly coexisting with peptide histidine isoleucine (PHI), vasoactive intestinal polypeptide (VIP) and neuropeptide tyrosine (NPY). In the pelvic ganglia a comparatively smaller proportion of neurons was NOS-positive and they often contained VIP-LI and less frequently NPY-LI. In the tissues innervated by these ganglia, such as nasal mucosa and salivary glands, NOS-positive fibers were observed around blood vessels and within the glandular parenchyma, although generally less abundant than VIP/PHI nerves, while in the uterus, vas deferens and penis a more close correlation was seen. NOS-positive fibers were also widely distributed in other tissues. In the sympathetic ganglia NOS-LI was mainly present in dense fiber networks, which disappeared after transection of the sympathetic trunc central to the ganglion. Since many cell bodies in the sympathetic lateral column of the spinal cord also were NOS-positive, it is likely that the majority of preganglionic fibers innervating sympathetic ganglia are NOS-positive. VIP-positive cells in stellate ganglia did not contain NOS-LI. The present results suggest that NO may be a messenger molecule both in parasympathetic postganglionic neurons and in preganglionic sympathetic neurons.
在本免疫组织化学研究中，对大鼠不同自主神经节及相关外周组织中一氧化氮合酶（NOS）的分布进行了研究。为作比较，在相邻或同一切片上分析了包括乙酰胆碱酯酶、酪氨酸羟化酶以及几种神经肽在内的其他一些神经元标志物。对NOS样免疫反应性（LI）及这些其他标志物的分布进行了半定量总结。在一些副交感神经节，如蝶腭神经节和下颌下神经节中，大多数神经节细胞存在NOS-LI，至少部分与肽组氨酸异亮氨酸（PHI）、血管活性肠肽（VIP）和神经肽Y（NPY）共存。在盆腔神经节中，NOS阳性神经元的比例相对较小，且它们常含有VIP-LI，较少含有NPY-LI。在这些神经节支配的组织，如鼻黏膜和唾液腺中，在血管周围和腺实质内观察到NOS阳性纤维，尽管其数量通常比VIP/PHI神经少，而在子宫、输精管和阴茎中则观察到更密切的相关性。NOS阳性纤维也广泛分布于其他组织中。在交感神经节中，NOS-LI主要存在于密集的纤维网络中，在神经节中枢侧交感干横断后这些网络消失。由于脊髓交感侧柱中的许多细胞体也呈NOS阳性，因此支配交感神经节的大多数节前纤维可能是NOS阳性的。星状神经节中的VIP阳性细胞不含有NOS-LI。目前的结果表明，NO可能是副交感神经节后神经元和交感神经节前神经元中的一种信使分子。
