Maesaki S, Kohno S, Kaku M, Koga H, Hara K
Second Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Antimicrob Agents Chemother. 1994 Dec;38(12):2843-5. doi: 10.1128/AAC.38.12.2843.
The in vitro effects of antifungal agent combinations administered simultaneously and sequentially against 15 strains of Aspergillus fumigatus were tested by the yeast-malt broth method. The synergistic effect of the combination of amphotericin B (AMPH) and miconazole was observed in nine strains (60%). However, the combinations of AMPH and fluconazole, AMPH and ketoconazole, and AMPH and itraconazole administered simultaneously showed antagonistic effects against three (20%), five (34%), and four (26%) strains, respectively. The effects of combinations of azole antifungal agents administered simultaneously were indifferent or antagonistic against A. fumigatus. In experiments measuring the effects of sequentially administered antifungal agents, however, pretreatment with AMPH and then azole antifungal agents exhibited better in vitro efficacy than that found in experiments measuring the effects of simultaneously administered AMPH and azole compounds.
采用酵母麦芽汁肉汤法，对同时和序贯给予抗真菌药物组合针对15株烟曲霉的体外作用进行了测试。两性霉素B（AMPH）和咪康唑联合使用时，在9株菌株（60%）中观察到协同作用。然而，同时给予AMPH和氟康唑、AMPH和酮康唑以及AMPH和伊曲康唑的组合，分别对3株（20%）、5株（34%）和4株（26%）菌株显示出拮抗作用。同时给予唑类抗真菌药物组合对烟曲霉的作用无差异或呈拮抗作用。然而，在测量序贯给予抗真菌药物作用的实验中，先用AMPH预处理然后再用唑类抗真菌药物，其体外疗效优于测量同时给予AMPH和唑类化合物作用的实验。
