Schabert F A, Engel A
Maurice E.-Müller Institute for Microscopic Structure Biology Biozentrum, University of Basel, Switzerland.
Biophys J. 1994 Dec;67(6):2394-403. doi: 10.1016/S0006-3495(94)80726-X.
Crystalline membranes reconstituted from Escherichia coli OmpF porin and phospholipids were adsorbed to freshly cleaved mica and imaged in solution by the atomic force microscope. The extracellular as well as the periplasmic side of the porin trimers could be identified and the conditions to record topographs at 1-nm lateral and 0.1-nm vertical resolution were established.
由大肠杆菌OmpF孔蛋白和磷脂重构的晶体膜被吸附到新劈开的云母上，并通过原子力显微镜在溶液中成像。可以识别孔蛋白三聚体的细胞外侧以及周质侧，并确定了以1纳米横向分辨率和0.1纳米垂直分辨率记录形貌图的条件。
