Frieauff W, Romagna F
Sandoz Pharma Ltd, Basle, Switzerland.
Cell Biol Toxicol. 1994 Dec;10(5-6):283-9. doi: 10.1007/BF00755771.
The mouse bone marrow micronucleus assay is an in vivo test commonly used in the pharmaceutical industry to evaluate the genotoxic potential of new compounds. The test detects agent-induced chromosomal damage or damage of the mitotic spindle apparatus. In this paper the state-of-the-art in automated rodent micronucleus evaluation using computerized image analysis in combination with high-quality slides obtained by the cellulose column fractionation technique is reviewed. The latter allows the effective removal of nucleated cells from rodent bone marrow. It has been found that automatic micronucleus scoring with the Leitz MIAC image analyzer is substantially faster than labor-intensive manual analysis. Automatic scoring can be performed overnight for up to 16 slides. We have been successfully using automatic micronucleus analysis for the testing of new pharmaceutical drugs for more than 3 years.
小鼠骨髓微核试验是制药行业常用的一种体内试验，用于评估新化合物的遗传毒性潜力。该试验可检测试剂诱导的染色体损伤或有丝分裂纺锤体装置的损伤。本文综述了利用计算机图像分析结合纤维素柱分级分离技术获得的高质量玻片进行自动化啮齿动物微核评估的最新进展。后者能有效去除啮齿动物骨髓中的有核细胞。已发现使用徕卡MIAC图像分析仪进行自动微核评分比劳动强度大的手动分析要快得多。自动评分可在一夜之间对多达16张玻片进行。我们成功使用自动微核分析检测新的制药药物已有3年多时间。
