Champiat D, Roux A, Lhomme O, Nosenzo G
MDC System, La Nitière, Neuville sur Ain, France.
Cell Biol Toxicol. 1994 Dec;10(5-6):345-51. doi: 10.1007/BF00755781.
Although used for analytical purposes for more than 40 years it is only recently that biochemiluminescence (BCL) has found widespread acceptance. Methods employing BCL reactions now play an important role in biomedical research and laboratory medicine. The main attractions for the assay technology include exquisite sensitivity (attomole-zeptomole), high selectivity, speed and simplicity. In biomedical research, the most important applications of BCL are: (1) to estimate microbial numbers and to assess cellular states (e.g., after exposure to antibiotic or cytotoxic agents) and in reporter gene studies (firefly luciferase gene); (2) NAD(P)H involved in redox/dehydrogenase studies using Vibrio luciferase complex; (3) BCL labels and CL detection of enzyme labels in immunoassays are the most widespread routine application for this technology. BCL enzyme immunoassays represent the most active area of development, e.g., enhanced BCL method for peroxidase and BCL assays for alkaline phosphatase labels using adamantyl 1,2-dioxetane.
尽管生物化学发光（BCL）用于分析目的已有40多年，但直到最近它才得到广泛认可。采用BCL反应的方法现在在生物医学研究和检验医学中发挥着重要作用。该检测技术的主要优点包括极高的灵敏度（阿托摩尔-zeptomole）、高选择性、速度快和操作简单。在生物医学研究中，BCL的最重要应用包括：（1）估计微生物数量和评估细胞状态（例如，在暴露于抗生素或细胞毒性剂之后）以及在报告基因研究（萤火虫荧光素酶基因）中；（2）在使用费氏弧菌荧光素酶复合物的氧化还原/脱氢酶研究中涉及的NAD(P)H；（3）BCL标记以及免疫分析中酶标记的化学发光检测是该技术最广泛的常规应用。BCL酶免疫分析是最活跃的发展领域，例如，用于过氧化物酶的增强型BCL方法以及使用金刚烷基1,2 - 二氧杂环丁烷对碱性磷酸酶标记物进行的BCL分析。
