Chen C S, Bejcek B E, Kersey J H
Department of Laboratory Medicine/Pathology, University of Minnesota, Minneapolis 55455, USA.
Cytogenet Cell Genet. 1995;69(3-4):260-5. doi: 10.1159/000133976.
A chromosome band 4q21 gene (MLLT2, formerly called AF-4/FEL) involved in a reciprocal translocation with chromosome band 11q23 in t(4;11) acute leukemia has been cloned. To provide better definition of gene order and relationships in this region where MLLT2 resides, we used pulsed field gel electrophoresis (PFGE) to investigate 13 genes (including MLLT2) with physical locations in bands 4q11-->q25. Somatic cell hybrids derived from RS4;11, a leukemic cell line carrying the t(4;11)(q21;q23), were also used to localize genes in relation to MLLT2. Linkage of the interleukin 8 (IL8), albumin (ALB), and platelet factor 4 (PF4) genes was confirmed by NotI, SalI and SacII digests. The maximum distance between PF4 and ALB is 210 kb and between ALB and IL8 is 420 kb. The alcohol dehydrogenase, class I (ADH2, ADH3) gene cluster can be linked to the alcohol dehydrogenase, class III gene (ADH5) by SacII, NruI, and EagI digests. The maximum distance between them is 590 kb. Our study indicated that ALB, alpha-fetoprotein (AFP), PF4, beta-thromboglobulin (PPBP), GRO1 (encoding a cytokine also called melanoma growth-stimulatory activity), and IL8 genes can be physically linked. In this study the gamma-interferon induced protein 10 (INP10), bone morphogenetic protein 3 (BMP3), annexin III (ANX3), KIT, amphiregulin (AREG), immunoglobulin J polypeptide (IGJ), deoxycytidine kinase (DCK) and MLLT2 genes were not linked to one another or to the above two groups of genes. Our analysis using somatic cell hybrids combined with previous reports demonstrated that the ADH gene cluster is telomeric to MLLT2 and KIT, ALB, AFP, PF4, beta TG, GRO1, IL8, ANX3, AREG and DCK are centromeric to MLLT2.
一个与t(4;11)急性白血病中11号染色体23带发生相互易位的4号染色体21带基因（MLLT2，以前称为AF-4/FEL）已被克隆。为了更清晰地界定MLLT2所在区域的基因顺序及相互关系，我们使用脉冲场凝胶电泳（PFGE）研究了13个基因（包括MLLT2），这些基因在4号染色体11带至25带区域有物理定位。源自RS4;11（一种携带t(4;11)(q21;q23)的白血病细胞系）的体细胞杂种也被用于确定与MLLT2相关的基因定位。通过NotI、SalI和SacII酶切证实了白细胞介素8（IL8）、白蛋白（ALB）和血小板因子4（PF4）基因的连锁关系。PF4与ALB之间的最大距离为210 kb，ALB与IL8之间的最大距离为420 kb。通过SacII、NruI和EagI酶切可将I类乙醇脱氢酶（ADH2、ADH3）基因簇与III类乙醇脱氢酶（ADH5）基因相连，它们之间的最大距离为590 kb。我们的研究表明，ALB、甲胎蛋白（AFP）、PF4、β-血小板球蛋白（PPBP）、GRO1（编码一种也称为黑色素瘤生长刺激活性的细胞因子）和IL8基因在物理上是相连的。在本研究中，γ-干扰素诱导蛋白10（INP10）、骨形态发生蛋白3（BMP3）、膜联蛋白III（ANX3）、KIT、双调蛋白（AREG）、免疫球蛋白J多肽（IGJ）、脱氧胞苷激酶（DCK）和MLLT2基因彼此之间或与上述两组基因均无连锁关系。我们利用体细胞杂种进行的分析结合先前的报道表明，ADH基因簇位于MLLT2的端粒侧，而KIT、ALB、AFP、PF4、β TG、GRO1、IL8、ANX3、AREG和DCK位于MLLT2的着丝粒侧。
