粟酒裂殖酵母ERG24同源物的克隆与序列分析。

Cloning and sequence analysis of an ERG24 homolog from Schizosaccharomyces pombe.

作者信息

Smith S

机构信息

Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021, USA.

出版信息

Gene. 1995 Mar 21;155(1):139-40. doi: 10.1016/0378-1119(94)00902-5.

DOI:10.1016/0378-1119(94)00902-5

PMID:7698661

Abstract

The Schizosaccharomyces pombe (Sp) erg24 cDNA, encoding C-14 sterol reductase (erg24p), has been cloned and sequenced. The nucleotide sequence of Sp erg24 contains an open reading frame encoding a 424-amino-acid protein. The deduced aa sequence of Sp erg24 shows significant homology with Saccharomyces cerevisiae (Sc) Erg24p, as well as with other members of a larger gene family that includes yeast C-24(28) sterol reductase (Erg4p) and a vertebrate inner nuclear membrane protein, the lamin B receptor (LBR).

摘要

编码C-14固醇还原酶（erg24p）的粟酒裂殖酵母（Sp）erg24 cDNA已被克隆并测序。Sp erg24的核苷酸序列包含一个编码424个氨基酸蛋白质的开放阅读框。Sp erg24推导的氨基酸序列与酿酒酵母（Sc）Erg24p以及一个更大基因家族的其他成员具有显著同源性，该基因家族包括酵母C-24(28)固醇还原酶（Erg4p）和一种脊椎动物内核膜蛋白——核纤层蛋白B受体（LBR）。