Oakey H J, Harty D W, Knox K W
Institute of Dental Research, Surry Hills, Sydney, NSW, Australia.
J Appl Bacteriol. 1995 Feb;78(2):142-8. doi: 10.1111/j.1365-2672.1995.tb02834.x.
Fifty-six strains of lactobacilli were examined for the production of glycosidases and proteases (arylamidases) that could be associated with the ability to grow in vivo and/or be a factor in the pathogenesis of endocarditis. The strains were from seven species, with an emphasis on Lactobacillus rhamnosus and Lact. paracasei subsp. paracasei, both of which have been associated with endocarditis and provided 12 of the 13 strains isolated from cases of the disease. Other species were Lact. acidophilus, Lact. plantarum, Lact. salivarius, Lact. fermentum and Lact. oris. Commonly expressed glycosidase activities were alpha-D-galactosidase and beta-N-acetyl-D-glucosaminidase followed by beta-D-glucosidase and alpha-L-fucosidase. The combined production of beta-N-acetyl-D-glucosaminidase and alpha-D-galactosidase was a feature of the endocarditis isolates. In contrast, beta-D-galactosidase was produced by very few of the strains within species implicated in endocarditis but most of the strains of Lact. salivarius, Lact. fermentum and Lact. oris. The most commonly produced arylamidases active against substrates employed for testing human blood clotting cascade were activated protein C(Ca)-like, activated factor X(Xa)-like and Hageman factor-like followed by kallikrein-like and chymotrypsin-like enzymes.(ABSTRACT TRUNCATED AT 250 WORDS)
检测了56株乳酸菌的糖苷酶和蛋白酶（芳基酰胺酶）的产生情况，这些酶可能与体内生长能力及/或心内膜炎发病机制中的一个因素有关。这些菌株来自七个物种，重点是鼠李糖乳杆菌和副干酪乳杆菌副干酪亚种，这两个物种都与心内膜炎有关，并且提供了从该疾病病例中分离出的13株菌株中的12株。其他物种包括嗜酸乳杆菌、植物乳杆菌、唾液乳杆菌、发酵乳杆菌和口乳杆菌。常见的糖苷酶活性为α-D-半乳糖苷酶和β-N-乙酰-D-葡糖胺酶，其次是β-D-葡萄糖苷酶和α-L-岩藻糖苷酶。β-N-乙酰-D-葡糖胺酶和α-D-半乳糖苷酶的联合产生是心内膜炎分离株的一个特征。相比之下，在心内膜炎相关物种中的极少数菌株产生β-D-半乳糖苷酶，但唾液乳杆菌、发酵乳杆菌和口乳杆菌的大多数菌株产生该酶。对用于检测人体凝血级联反应的底物具有活性的最常见芳基酰胺酶是活化蛋白C（Ca）样、活化因子X（Xa）样和哈格曼因子样，其次是激肽释放酶样和胰凝乳蛋白酶样酶。（摘要截断于250字）
