Millemann Y, Lesage M C, Chaslus-Dancla E, Lafont J P
Institut National de la Recherche Agronomique, Centre de Tours-Nouzilly, Monnaie, France.
J Clin Microbiol. 1995 Jan;33(1):173-9. doi: 10.1128/jcm.33.1.173-179.1995.
Seventy selected strains of Salmonella typhimurium and S. enteritidis isolated from related poultry flocks in three independent geographical areas were characterized by phenotypic and genotypic methods to compare the usefulness of the methods in epidemiological studies. The 56 S. typhimurium isolates were poorly discriminated by their biotypes, resistance patterns, and plasmid profiles. Nine different ribotypes were obtained after DNA digestion by BglII, PvuII, and SmaI. Seven IS200 types, characterized by six to nine copies of IS200 on the chromosome, were detected after digestion of genomic DNA by PstI. These studies resulted in the definition of 15 clonal lineages distributed in three clusters. The 14 S. enteritidis strains were not discriminated either by ribotyping or by detection of IS200 (IS200 typing), but were separated on the basis of antibiotic resistance and plasmid profiling. The stability of the insertion sequence type was confirmed by inoculation of an S. typhimurium strain to axenic chickens reared for 15 weeks in sterile isolators.
从三个独立地理区域的相关家禽群中分离出70株鼠伤寒沙门氏菌和肠炎沙门氏菌，通过表型和基因型方法对其进行特征分析，以比较这些方法在流行病学研究中的实用性。56株鼠伤寒沙门氏菌分离株通过生物型、耐药模式和质粒图谱难以区分。用BglII、PvuII和SmaI消化DNA后获得了9种不同的核糖型。用PstI消化基因组DNA后，检测到7种IS200类型，其特征是染色体上有6至9个IS200拷贝。这些研究确定了分布在三个簇中的15个克隆谱系。14株肠炎沙门氏菌菌株通过核糖分型或IS200检测（IS200分型）无法区分，但根据抗生素耐药性和质粒图谱进行了分离。通过将一株鼠伤寒沙门氏菌菌株接种到在无菌隔离器中饲养15周的无菌鸡中，证实了插入序列类型的稳定性。
