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大肠杆菌中接合中断的简化方法。

Simplified method for interruption of conjugation in Escherichia coli.

作者信息

Zipkas D, Riley M

出版信息

J Bacteriol. 1976 Apr;126(1):559-60. doi: 10.1128/jb.126.1.559-560.1976.

DOI:10.1128/jb.126.1.559-560.1976
PMID:770441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC233321/
Abstract

A simplified procedure is described in which interruption of mating and recombinant selection both take place on plates containing selective medium plus nalidixic acid.

摘要

本文描述了一种简化程序，其中交配中断和重组体选择均在含有选择性培养基加萘啶酸的平板上进行。

相似文献

1
Simplified method for interruption of conjugation in Escherichia coli.大肠杆菌中接合中断的简化方法。
J Bacteriol. 1976 Apr;126(1):559-60. doi: 10.1128/jb.126.1.559-560.1976.
2
Loss of Hfr DNA from Escherichia coli merozygotes during inhibition of conjugation by nalidixic acid.
Genet Res. 1980 Aug;36(1):69-79. doi: 10.1017/s0016672300019674.
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[Simplified method for conjugational transference of R factors on solid culture media].[R因子在固体培养基上结合转移的简易方法]
Antibiotiki. 1971 Feb;16(2):158-62.
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Nalidixic acid inhibition of DNA transfer in Escherichia coli K12.萘啶酸对大肠杆菌K12中DNA转移的抑制作用。
Cold Spring Harb Symp Quant Biol. 1968;33:629-33. doi: 10.1101/sqb.1968.033.01.070.
5
Formation of transferable drug resistance factor by recombination between resistance determinants and transfer factors.
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Mechanism of action of nalidixic acid on conjugating bacteria.萘啶酸对结合细菌的作用机制。
J Bacteriol. 1970 Jun;102(3):688-701. doi: 10.1128/jb.102.3.688-701.1970.
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On the mechanism of conjugation in Escherichia coli K 12.关于大肠杆菌K12中接合作用的机制
Mol Gen Genet. 1968;102(4):269-74. doi: 10.1007/BF00433718.
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Anaerobic R factor transfer in Escherichia coli.大肠杆菌中厌氧R因子的转移
J Gen Microbiol. 1973 Jun;76(2):437-40. doi: 10.1099/00221287-76-2-437.
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Mechanism of conjugation and recombination in bacteria. XX, Recombination DNA synthesis during mating in Escherichia coli K-12.细菌中接合与重组的机制。XX. 大肠杆菌K-12交配过程中的重组DNA合成。
Acta Microbiol Pol. 1979;28(1):15-27.
10
Effect of nalidixic acid on conjugational transfer and expression of episomal lac genes in Escherichia coli K12.萘啶酸对大肠杆菌K12中附加体lac基因的接合转移及表达的影响。
J Mol Biol. 1967 Sep 14;28(2):373-6. doi: 10.1016/s0022-2836(67)80016-0.

引用本文的文献

1
Amber mutation affecting the length of Escherichia coli cells.影响大肠杆菌细胞长度的琥珀突变
J Bacteriol. 1980 Nov;144(2):532-41. doi: 10.1128/jb.144.2.532-541.1980.
2
Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
Microbiol Rev. 1980 Mar;44(1):1-56. doi: 10.1128/mr.44.1.1-56.1980.
3
Genetic characterization of the Escherichia coli cyclopropane fatty acid (cfa) locus and neighboring loci.大肠杆菌环丙烷脂肪酸（cfa）基因座及相邻基因座的遗传特征分析。
Mol Gen Genet. 1984;196(2):367-72. doi: 10.1007/BF00328074.
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The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa.铜绿假单胞菌中精氨酸生物合成的基因组织。
Mol Gen Genet. 1977 Jul 7;154(1):7-22. doi: 10.1007/BF00265571.
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Isolation and properties of Escherichia coli K-12 mutants impaired in the utilization of gamma-aminobutyrate.γ-氨基丁酸利用受损的大肠杆菌K-12突变体的分离与特性
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A mutation that increases the activity of nonsense suppressors in Escherichia coli.一种增加大肠杆菌中无义抑制因子活性的突变。
Mol Gen Genet. 1978 Feb 16;159(2):161-9. doi: 10.1007/BF00270889.
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Protection of foreign DNA against host-controlled restriction in bacterial cells. I. protection of F' plasmid DNA by preinfection with bacteriophages T3 or T7.
Mol Gen Genet. 1978 Feb 7;159(1):107-10. doi: 10.1007/BF00401754.
8
Mutation affecting thermostability of sigma subunit of Escherichia coli RNA polymerase lies near the dnaG locus at about 66 min on the E. coli genetic map.影响大肠杆菌RNA聚合酶σ亚基热稳定性的突变位于大肠杆菌遗传图谱上约66分钟处的dnaG位点附近。
Proc Natl Acad Sci U S A. 1978 Jan;75(1):427-31. doi: 10.1073/pnas.75.1.427.
9
F plasmid genes involved in the production of recombination-stimulating factor, control of sensitivity to some injurious agents, and chromosome replication in Escherichia coli K-12 HfrC.参与重组刺激因子产生、对某些损伤因子敏感性控制以及大肠杆菌K-12 HfrC中染色体复制的F质粒基因。
J Bacteriol. 1978 Jan;133(1):33-42. doi: 10.1128/jb.133.1.33-42.1978.

本文引用的文献

1
A QUICK AND EFFICIENT METHOD FOR INTERRUPTION OF BACTERIAL CONJUGATION.一种快速有效的细菌接合阻断方法。
Genet Res. 1965 Jul;6:300-3. doi: 10.1017/s001667230000416x.
2
The kinetics of the mating process in Escherichia coli.大肠杆菌中交配过程的动力学
J Gen Microbiol. 1957 Feb;16(1):97-119. doi: 10.1099/00221287-16-1-97.
3
Nalidixic acid inhibition of DNA transfer in Escherichia coli K12.萘啶酸对大肠杆菌K12中DNA转移的抑制作用。
Cold Spring Harb Symp Quant Biol. 1968;33:629-33. doi: 10.1101/sqb.1968.033.01.070.
4
Molecular nature of two nonconjugative plasmids carrying drug resistance genes.携带耐药基因的两种非接合质粒的分子特性
J Bacteriol. 1974 Feb;117(2):619-30. doi: 10.1128/jb.117.2.619-630.1974.
5
Rapid mapping of conditional and auxotrophic mutations in Escherichia coli K-12.大肠杆菌K-12中条件性突变和营养缺陷型突变的快速定位
J Bacteriol. 1973 Feb;113(2):798-812. doi: 10.1128/jb.113.2.798-812.1973.