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In vivo detection of radicals in biological reactions.生物反应中自由基的体内检测。
Environ Health Perspect. 1994 Dec;102 Suppl 10(Suppl 10):29-32. doi: 10.1289/ehp.94102s1029.
2
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引用本文的文献

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The quantitative measurement of H2O2 generation in isolated mitochondria.分离线粒体中过氧化氢生成的定量测定。
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本文引用的文献

1
Chemiluminescent response to PMA in isolated rat liver after in situ ischemia-reperfusion.
Free Radic Biol Med. 1993 Dec;15(6):575-80. doi: 10.1016/0891-5849(93)90159-r.
2
Direct measurement of free radical generation following reperfusion of ischemic myocardium.缺血心肌再灌注后自由基生成的直接测量。
Proc Natl Acad Sci U S A. 1987 Mar;84(5):1404-7. doi: 10.1073/pnas.84.5.1404.
3
NMR detection of creatine kinase expressed in liver of transgenic mice: determination of free ADP levels.核磁共振检测转基因小鼠肝脏中表达的肌酸激酶：游离ADP水平的测定
Proc Natl Acad Sci U S A. 1990 Apr;87(8):3112-6. doi: 10.1073/pnas.87.8.3112.
4
Glutathione and ischemia-reperfusion injury in the perfused rat liver.谷胱甘肽与灌注大鼠肝脏的缺血再灌注损伤
Free Radic Biol Med. 1992;12(4):271-9. doi: 10.1016/0891-5849(92)90114-v.
5
Phorbol myristate acetate-induced lung injury: involvement of reactive oxygen species.佛波醇肉豆蔻酸酯乙酸酯诱导的肺损伤：活性氧的作用
Scand J Clin Lab Invest. 1992 Nov;52(7):753-61. doi: 10.3109/00365519209115522.

生物反应中自由基的体内检测。

In vivo detection of radicals in biological reactions.

作者信息

Chance B, Gao G

机构信息

Department of Biochemistry and Biophysics, Johnson Foundation, University of Pennsylvania, Philadelphia 19104.

出版信息

Environ Health Perspect. 1994 Dec;102 Suppl 10(Suppl 10):29-32. doi: 10.1289/ehp.94102s1029.

DOI:10.1289/ehp.94102s1029
PMID:7705300
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1566976/
Abstract

In vivo detection of free radicals on a quantitative basis is essential for an establishment of their role in membrane, protein, and cell and tissue damage. This article compares optical methods, including chemiluminescence, electron paramagnetic resonance (EPR) and NMR methods for the study of free radicals and free radical damage, especially reperfusion injury neutrophil accumulation 6 hr after the ischemia/reperfusion insult. The chemiluminescence method is applied to an evaluation of the improved resistance to free radical stress due to transfection of creatine kinase in the mouse liver.

摘要

在定量基础上对体内自由基进行检测，对于确定它们在膜、蛋白质以及细胞和组织损伤中的作用至关重要。本文比较了光学方法，包括化学发光法、电子顺磁共振（EPR）和核磁共振方法，用于研究自由基和自由基损伤，特别是缺血/再灌注损伤6小时后中性粒细胞积聚导致的再灌注损伤。化学发光法用于评估小鼠肝脏中由于肌酸激酶转染而对自由基应激的抗性增强情况。