利用电子自旋共振检测生物系统中的氧衍生自由基。
Detection of oxygen-derived radicals in biological systems using electron spin resonance.
作者信息
Mason R P, Hanna P M, Burkitt M J, Kadiiska M B
机构信息
Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.
出版信息
Environ Health Perspect. 1994 Dec;102 Suppl 10(Suppl 10):33-6. doi: 10.1289/ehp.94102s1033.
Abstract
Oxygen-centered radicals, particularly the hydroxyl and superoxide radicals, have been postulated in many biochemical reactions and have been implicated in many adverse reactions in vivo. This article begins with a review of spin-trapping detection of oxygen-centered radicals in vitro and concludes with a presentation of our approach to the detection of the hydroxyl radicals in models of acute iron and copper poisoning.
摘要
以氧为中心的自由基，特别是羟基自由基和超氧阴离子自由基，已在许多生化反应中被推测存在，并与体内许多不良反应有关。本文首先回顾了体外以氧为中心的自由基的自旋捕捉检测，最后介绍了我们在急性铁和铜中毒模型中检测羟基自由基的方法。
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本文引用的文献
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In vivo ESR spin trapping evidence for hydroxyl radical-mediated toxicity of paraquat and copper in rats.体内电子自旋共振自旋捕获法证明百草枯和铜对大鼠的毒性由羟自由基介导
Toxicol Appl Pharmacol. 1993 Dec;123(2):187-92. doi: 10.1006/taap.1993.1236.
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Detection of superoxide generated by endothelial cells.内皮细胞产生的超氧化物的检测。
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[17O]oxygen hyperfine structure for the hydroxyl and superoxide radical adducts of the spin traps DMPO, PBN and 4-POBN.自旋捕获剂DMPO、PBN和4-POBN的羟基和超氧自由基加合物的[17O]氧超精细结构
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Direct evidence for in vivo hydroxyl-radical generation in experimental iron overload: an ESR spin-trapping investigation.实验性铁过载体内羟基自由基生成的直接证据：一项电子顺磁共振自旋捕获研究。
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