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利用电子自旋共振检测生物系统中的氧衍生自由基。

Detection of oxygen-derived radicals in biological systems using electron spin resonance.

作者信息

Mason R P, Hanna P M, Burkitt M J, Kadiiska M B

机构信息

Laboratory of Molecular Biophysics, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709.

出版信息

Environ Health Perspect. 1994 Dec;102 Suppl 10(Suppl 10):33-6. doi: 10.1289/ehp.94102s1033.

DOI:10.1289/ehp.94102s1033
PMID:7705301
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1566981/
Abstract

Oxygen-centered radicals, particularly the hydroxyl and superoxide radicals, have been postulated in many biochemical reactions and have been implicated in many adverse reactions in vivo. This article begins with a review of spin-trapping detection of oxygen-centered radicals in vitro and concludes with a presentation of our approach to the detection of the hydroxyl radicals in models of acute iron and copper poisoning.

摘要

以氧为中心的自由基，特别是羟基自由基和超氧阴离子自由基，已在许多生化反应中被推测存在，并与体内许多不良反应有关。本文首先回顾了体外以氧为中心的自由基的自旋捕捉检测，最后介绍了我们在急性铁和铜中毒模型中检测羟基自由基的方法。

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1
Detection of oxygen-derived radicals in biological systems using electron spin resonance.利用电子自旋共振检测生物系统中的氧衍生自由基。
Environ Health Perspect. 1994 Dec;102 Suppl 10(Suppl 10):33-6. doi: 10.1289/ehp.94102s1033.
2
Electron spin resonance spin-trapping investigation into the effects of paraquat and desferrioxamine on hydroxyl radical generation during acute iron poisoning.电子自旋共振自旋捕集法研究百草枯和去铁胺对急性铁中毒时羟自由基生成的影响。
Mol Pharmacol. 1993 Feb;43(2):257-63.
3
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本文引用的文献

1
Electron spin resonance spin-trapping investigation into the effects of paraquat and desferrioxamine on hydroxyl radical generation during acute iron poisoning.电子自旋共振自旋捕集法研究百草枯和去铁胺对急性铁中毒时羟自由基生成的影响。
Mol Pharmacol. 1993 Feb;43(2):257-63.
2
In vivo ESR spin trapping evidence for hydroxyl radical-mediated toxicity of paraquat and copper in rats.体内电子自旋共振自旋捕获法证明百草枯和铜对大鼠的毒性由羟自由基介导
Toxicol Appl Pharmacol. 1993 Dec;123(2):187-92. doi: 10.1006/taap.1993.1236.
3
Production of hydroxyl radical by decomposition of superoxide spin-trapped adducts.超氧化物自旋捕获加合物分解产生羟基自由基。
Mol Pharmacol. 1982 Mar;21(2):262-5.
4
Detection of superoxide generated by endothelial cells.内皮细胞产生的超氧化物的检测。
Proc Natl Acad Sci U S A. 1984 Dec;81(23):7269-73. doi: 10.1073/pnas.81.23.7269.
5
[17O]oxygen hyperfine structure for the hydroxyl and superoxide radical adducts of the spin traps DMPO, PBN and 4-POBN.自旋捕获剂DMPO、PBN和4-POBN的羟基和超氧自由基加合物的[17O]氧超精细结构
Biochem Biophys Res Commun. 1986 Dec 15;141(2):622-8. doi: 10.1016/s0006-291x(86)80218-2.
6
Paraquat uptake into freshly isolated rabbit lung epithelial cells and its reduction to the paraquat radical under anaerobic conditions.百草枯进入新鲜分离的兔肺上皮细胞及其在厌氧条件下还原为百草枯自由基的过程。
Mol Pharmacol. 1986 May;29(5):484-8.
7
Direct evidence for in vivo hydroxyl-radical generation in experimental iron overload: an ESR spin-trapping investigation.实验性铁过载体内羟基自由基生成的直接证据：一项电子顺磁共振自旋捕获研究。
Proc Natl Acad Sci U S A. 1991 Oct 1;88(19):8440-4. doi: 10.1073/pnas.88.19.8440.
8
Reduction of paraquat and related bipyridylium compounds to free radical metabolites by rat hepatocytes.大鼠肝细胞将百草枯及相关联吡啶鎓化合物还原为自由基代谢产物。
Arch Biochem Biophys. 1991 Aug 15;289(1):145-52. doi: 10.1016/0003-9861(91)90454-q.
9
Comparison of pediatric poisoning hazards: an analysis of 3.8 million exposure incidents. A report from the American Association of Poison Control Centers.儿童中毒危害比较：对380万起暴露事件的分析。美国中毒控制中心协会的一份报告。
Pediatrics. 1992 Jun;89(6 Pt 1):999-1006.
10
In vivo evidence of hydroxyl radical formation after acute copper and ascorbic acid intake: electron spin resonance spin-trapping investigation.急性摄入铜和抗坏血酸后体内羟基自由基形成的证据：电子自旋共振自旋捕集研究
Mol Pharmacol. 1992 Oct;42(4):723-9.