Tronnier M, Smolle J, Wolff H H
Department of Dermatology, Medical University of Lübeck, Germany.
J Invest Dermatol. 1995 Apr;104(4):475-8. doi: 10.1111/1523-1747.ep12605910.
Ultraviolet (UV) light represents one of the factors that might play a role in the initiation and promotion of malignant transformation of human melanocytes. To determine the short-term effects of UV irradiation on melanocytic nevi in vivo, we investigated one half of symmetric melanocytic nevi after a single UV exposure with double the patient's minimal erythema dose. This half was compared with the nonirradiated, shielded half of the same nevus. The different parts were examined histologically for differences and immunohistochemically for the presence of HMB-45 antigen and proliferating cell nuclear antigen. The features were assessed quantitatively by image analysis. One week after the single UV irradiation, we observed a significant increase of suprabasally located melanocytes and a markedly enhanced expression of HMB-45, whereas proliferative activity of the cells was unchanged. In nevi that were excised 2 or 3 weeks after irradiation, no significant differences were observed between the irradiated and the nonirradiated part. The results indicate that a single UV irradiation may induce transient melanocytic activation with morphologic and histologic changes. Although these data do not formally assess resemblance to melanoma, these changes may be similar to those of melanoma in situ.
紫外线（UV）是可能在人类黑素细胞恶性转化的起始和促进过程中发挥作用的因素之一。为了确定紫外线照射对体内黑素细胞痣的短期影响，我们用患者最小红斑量的两倍对对称黑素细胞痣的一半进行单次紫外线照射，然后将其与同一痣未照射、受保护的另一半进行比较。对不同部分进行组织学检查以发现差异，并进行免疫组织化学检查以检测HMB - 45抗原和增殖细胞核抗原的存在情况。通过图像分析对这些特征进行定量评估。单次紫外线照射一周后，我们观察到基底层上方黑素细胞显著增加，HMB - 45表达明显增强，而细胞的增殖活性未改变。在照射后2或3周切除的痣中，照射部分和未照射部分之间未观察到显著差异。结果表明，单次紫外线照射可能诱导黑素细胞短暂激活并伴有形态学和组织学变化。虽然这些数据未正式评估与黑色素瘤的相似性，但这些变化可能与原位黑色素瘤的变化相似。
