Hunzelmann N, Schönherr E, Bonnekoh B, Hartmann C, Kresse H, Krieg T
Department of Dermatology, University of Cologne, Germany.
J Invest Dermatol. 1995 Apr;104(4):509-13. doi: 10.1111/1523-1747.ep12605979.
Small proteoglycans have been shown to act as receptors for matrix molecules or growth factors and to influence the attachment and the migration of cells. We therefore report here on the immunocytochemical expression of three small proteoglycans, i.e., decorin, biglycan, and the recently described PG-100, in normal human skin and in basal cell carcinoma. In normal human skin, staining for decorin revealed expression throughout the dermis with an increased signal in the papillary dermis, whereas no expression was observed in the epidermis. Biglycan and PG-100 were mainly detected in the epidermis, with biglycan being expressed only in suprabasal layers. In addition, biglycan could be detected in a narrow zone below the basement membrane. In tissue specimens obtained from 12 basal cell carcinomas, the expression of biglycan and PG-100 was absent or strongly down-regulated in the tumor tissue. Tumor cells thus displayed a staining pattern similar to that found on the basal cells of normal human skin. In the stroma surrounding the tumor, however, the expression of biglycan and to a lesser degree decorin was increased when compared with normal human dermis. The increased deposition appears to be due to an increased synthesis of these molecules, as total RNA extracted from basal cell carcinoma tissue revealed an induction of biglycan and decorin mRNA. This study indicates that the expression of proteoglycans in basal cell carcinoma tumor cells and in tumor stroma is altered from that in normal skin.
小蛋白聚糖已被证明可作为基质分子或生长因子的受体，并影响细胞的附着和迁移。因此，我们在此报告三种小蛋白聚糖，即核心蛋白聚糖、双糖链蛋白聚糖和最近描述的PG - 100，在正常人类皮肤和基底细胞癌中的免疫细胞化学表达。在正常人类皮肤中，核心蛋白聚糖染色显示在整个真皮层均有表达，在乳头层信号增强，而在表皮中未观察到表达。双糖链蛋白聚糖和PG - 100主要在表皮中检测到，双糖链蛋白聚糖仅在基底上层表达。此外，在基底膜下方的狭窄区域可检测到双糖链蛋白聚糖。在取自12例基底细胞癌的组织标本中，肿瘤组织中双糖链蛋白聚糖和PG - 100的表达缺失或强烈下调。肿瘤细胞因此呈现出与正常人类皮肤基底细胞相似的染色模式。然而，与正常人类真皮相比，在肿瘤周围的基质中，双糖链蛋白聚糖的表达增加，核心蛋白聚糖的表达在较小程度上增加。沉积增加似乎是由于这些分子的合成增加，因为从基底细胞癌组织中提取的总RNA显示双糖链蛋白聚糖和核心蛋白聚糖mRNA的诱导。这项研究表明，基底细胞癌肿瘤细胞和肿瘤基质中蛋白聚糖的表达与正常皮肤中的表达有所不同。
