Dityatev A E, Kozhanov V M, Gapanovich S O, Clamann H P
Department of Physiology, University of Bern, Switzerland.
Pflugers Arch. 1994 Nov;429(1):22-6. doi: 10.1007/BF02584026.
A method for calculating quantal size based on spectral analysis of postsynaptic potential (PSP) amplitude distributions was developed and tested by Monte-Carlo techniques. It was combined with a test to reveal the bias of the estimate of quantal size and to determine whether the peaks in amplitude distributions reflect quantal release or a sampling artifact. Spectral density was computed using fast Fourier transformation after subtraction of a fitted polynomial from the probability density function. The method overestimated quantal size for values less than two standard deviations of noise, indicating that those estimates as well as estimates of quantal size computed for examples of non-quantal distributions are not reliable. This was the case for 34 of 36 sets of sensorimotor excitatory PSPs of the frog, suggesting that most values of the quanta in synapses between primary fibres and lumbar motoneurons are smaller than 70-90 microV.
一种基于突触后电位（PSP）幅度分布频谱分析来计算量子大小的方法被开发出来，并通过蒙特卡罗技术进行了测试。它与一项测试相结合，以揭示量子大小估计的偏差，并确定幅度分布中的峰值是反映量子释放还是抽样伪像。在从概率密度函数中减去拟合多项式后，使用快速傅里叶变换计算频谱密度。对于小于噪声两个标准差的值，该方法高估了量子大小，这表明这些估计以及为非量子分布示例计算的量子大小估计是不可靠的。青蛙的36组感觉运动兴奋性PSP中有34组是这种情况，这表明初级纤维与腰段运动神经元之间突触中量子的大多数值小于70 - 90微伏。
