细胞色素c阳离子的气相折叠与去折叠
Gas-phase folding and unfolding of cytochrome c cations.
作者信息
Wood T D, Chorush R A, Wampler F M, Little D P, O'Connor P B, McLafferty F W
机构信息
Baker Chemistry Laboratory, Cornell University, Ithaca, NY 14853-1301, USA.
出版信息
Proc Natl Acad Sci U S A. 1995 Mar 28;92(7):2451-4. doi: 10.1073/pnas.92.7.2451.
Abstract
Water is thought to play a dominant role in protein folding, yet gaseous multiply protonated proteins from which the water has been completely removed show hydrogen/deuterium (H/D) exchange behavior similar to that used to identify conformations in solution. Indicative of the gas-phase accessibility to D2O, multiply-charged (6+ to 17+) cytochrome c cations exchange at six (or more) distinct levels of 64 to 173 out of 198 exchangeable H atoms, with the 132 H level found at charge values 8+ to 17+. Infrared laser heating and fast collisions can apparently induce ions to unfold to exchange at a higher distinct level, while charge-stripping ions to lower charge values yields apparent folding as well as unfolding.
摘要
水被认为在蛋白质折叠过程中起主要作用，然而，完全去除了水的气态多质子化蛋白质表现出的氢/氘（H/D）交换行为，与用于识别溶液中构象的行为相似。多电荷（6+至17+）细胞色素c阳离子对D2O的气相可及性表明，在198个可交换氢原子中，有64至173个以六个（或更多）不同水平进行交换，在电荷值为8+至17+时发现132H水平。红外激光加热和快速碰撞显然可以诱导离子展开，以在更高的不同水平进行交换，而将离子电荷剥离至较低电荷值会产生明显的折叠以及展开。
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