Pandya P P, Cardy D L, Jauniaux E, Campbell S, Nicolaides K H
Harris Birthright Research Centre for Fetal Medicine, King's College Hospital Medical School, London, UK.
Fetal Diagn Ther. 1995 Jan-Feb;10(1):66-70. doi: 10.1159/000264195.
Coelomic fluid (n = 32), amniotic fluid (n = 26) and placental tissue were obtained from 32 women undergoing termination of pregnancy at 7-11 weeks of gestation. Fluorescence in situ hybridisation (FISH) was performed to determine fetal sex using a heterochromatic Y probe and an alpha satellite repeat probe for chromosome X. In each case there was concordance in the fetal sex from the three compartments. However, no result could be obtained from 4 of the 32 coelomic fluids and 4 of the 26 amniotic fluid samples. The hybridisation efficiency was significantly lower in coelomic and amniotic fluid compared with placental tissue.
从32名妊娠7至11周接受终止妊娠的女性中获取了体腔液（n = 32）、羊水（n = 26）和胎盘组织。使用异染色质Y探针和染色体X的α卫星重复探针进行荧光原位杂交（FISH）以确定胎儿性别。在每种情况下，三个样本中的胎儿性别结果一致。然而，32份体腔液样本中有4份以及26份羊水样本中有4份未能得出结果。与胎盘组织相比，体腔液和羊水的杂交效率显著较低。
