Gasc A M, Geslin P, Sicard A M
Laboratoire de Microbiologie et Génétique Moléculaire, CNRS, Toulouse, France.
Microbiology (Reading). 1995 Mar;141 ( Pt 3):623-7. doi: 10.1099/13500872-141-3-623.
Pulsed-field gel electrophoresis of the genomic DNA of penicillin-resistant strains of Streptococcus pneumoniae was carried out. Eleven clinical strains of serogroup 9 from different French towns and Paris hospitals were tested. The restriction enzymes Apal and Smal were used to digest intact chromosomes, and the fragments were resolved by field-inversion gel electrophoresis (FIGE). Five strains were similar using Apal and Smal. Four others were closely related when using Apal, and five others were closely related when using Smal. These results suggest that 10 of these strains are genetically related and have a clonal origin. The profile of the eleventh strain was completely different. Thus, in a given serotype the spreading of penicillin resistance can result from both clonal and independent events. Five strains had similar FIGE profiles to strains first isolated in Spain, suggesting that a resistant strain had spread from Spain to France.
对肺炎链球菌青霉素耐药菌株的基因组DNA进行了脉冲场凝胶电泳分析。检测了来自法国不同城镇和巴黎医院的11株9血清群临床菌株。使用限制性内切酶Apal和Smal消化完整的染色体，并通过场反转凝胶电泳（FIGE）分离片段。使用Apal和Smal时，有5株菌株相似。使用Apal时，另外4株密切相关；使用Smal时，另外5株密切相关。这些结果表明，其中10株菌株具有遗传相关性且起源于克隆。第11株菌株的图谱完全不同。因此，在给定血清型中，青霉素耐药性的传播可能源于克隆事件和独立事件。5株菌株的FIGE图谱与最初在西班牙分离的菌株相似，表明一株耐药菌株已从西班牙传播至法国。
