[通过电子显微镜检查和聚合酶链反应技术检测阴道和宫颈人乳头瘤病毒]

[Detection of vaginal and cervical human papillomavirus by electronic microscope examination and polymerase chain reaction technique].

作者信息

Chen Q, Mai Y Y, Guo Y

机构信息

First teaching Hospital, Beijing Medical University.

出版信息

Zhonghua Fu Chan Ke Za Zhi. 1994 Oct;29(10):595-7, 636.

PMID:7712872

Abstract

UNLABELLED

Forty-seven specimens, biopsied during April-July 1990, pathologically proven to be vaginal or cervical condyloma acuminata (CA) in 34, wart-like lesions in 6, infections in 7, were screened for detection of human papillomavirus (HPV) by electronic microscope (EM) examination and polymerase chain reaction (PCR) technique. HPV type 11 (HPV-11) infections were diagnosed by Dot-blot hybridization in 40 out of these 47 specimens.

RESULTS

(1) No typical virus particles was observed in all samples under EM examinations. However, interchromatin and perichromatin granules were found within the nuclei of koilocytes in 88.23% of the vaginal CA specimens. Nuclear bodies were seen in 3 out of 27 vaginal CA and HPV11 positive specimens. Furthermore, koilocytosis was also seen in the basal and parabasal layers of vaginal mucosa. (2) The positive rate for detection of HPV11 and HPV6 DNA by PCR technique in vaginal CA specimens was 92.6%, while positive rate for HPV 11, 6, 16 and 18 DNA was only 3.7%.

摘要

未标记

1990年4月至7月间活检的47份标本，经病理证实34份为阴道或宫颈尖锐湿疣（CA），6份为疣状病变，7份为感染，通过电子显微镜（EM）检查和聚合酶链反应（PCR）技术筛查人乳头瘤病毒（HPV）。在这47份标本中的40份中通过斑点杂交诊断出11型人乳头瘤病毒（HPV-11）感染。

结果

（1）在电子显微镜检查下所有样本中均未观察到典型病毒颗粒。然而，在88.23%的阴道CA标本的挖空细胞的细胞核内发现了染色质间和染色质周围颗粒。在27份阴道CA和HPV11阳性标本中有3份可见核体。此外，在阴道黏膜的基底层和副基底层也可见挖空细胞形成。（2）PCR技术检测阴道CA标本中HPV11和HPV6 DNA的阳性率为92.6%，而HPV 11、6、16和18 DNA的阳性率仅为3.7%。