Saban R, Undem B J, Keith I M, Saban M R, Tengowski M W, Graziano F M, Bjorling D E
Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin-Madison 53706.
J Urol. 1994 Aug;152(2 Pt 1):544-9. doi: 10.1016/s0022-5347(17)32790-8.
The relative contributions of mucosal/submucosal and detrusor layers to the release of inflammatory mediators were investigated in isolated, ovalbumin (OVA) sensitized guinea pig urinary bladders. Ovalbumin challenge of sensitized mucosa induced release of prostaglandins (PG): PGE2, PGD2 and PGF2 alpha, in that order of magnitude. The total release of PGs was significantly higher from the mucosa/submucosa than from the detrusor/serosa. Under the same conditions, net release of leukotriene (LT) was observed predominantly from the detrusor. The total amount of histamine released from the mucosa was greater than that from the muscle layer. These results indicate differential production and release of inflammatory mediators from the mucosal/submucosal and detrusor smooth muscle layers. These results may have serious implications in disorders, such as interstitial cystitis, involving bladder mucosal damage. The cytoprotective effect of PGE2 is likely to be lost when the mucosa is damaged, and LT release from deeper layers may contribute significantly to symptoms of bladder inflammation.
在分离的、经卵清蛋白（OVA）致敏的豚鼠膀胱中，研究了黏膜/黏膜下层和逼尿肌层对炎症介质释放的相对贡献。用卵清蛋白刺激致敏的黏膜会诱导前列腺素（PG）的释放：前列腺素E2（PGE2）、前列腺素D2（PGD2）和前列腺素F2α（PGF2α），其释放量依次递减。黏膜/黏膜下层释放的PG总量显著高于逼尿肌/浆膜层。在相同条件下，白三烯（LT）的净释放主要来自逼尿肌。从黏膜释放的组胺总量大于肌肉层。这些结果表明黏膜/黏膜下层和逼尿肌平滑肌层产生和释放炎症介质存在差异。这些结果可能对诸如间质性膀胱炎等涉及膀胱黏膜损伤的疾病具有严重影响。当黏膜受损时，PGE2的细胞保护作用可能会丧失，而深层释放的LT可能对膀胱炎症症状有显著影响。
