Gannon K S, Contreras R J
Department of Psychology, Florida State University, Tallahassee 32306-1051, USA.
Physiol Behav. 1995 Feb;57(2):231-9. doi: 10.1016/0031-9384(94)00279-e.
Mice of 129/J and C57BL/6J inbred strains received two-bottle, 48-h preference tests of NaCl solutions vs. distilled water. 129/J mice exhibited a greater preference for 0.08 M NaCl than did C57BL/6J mice. To determine if this strain difference was mediated by taste, the integrated neural responses of the chorda tympani nerve to a concentration range of NaCl and KCl were examined. Gustatory neural responses to NaCl were similar for 129/J and C57BL/6J mice. However, lingual application of 0.5 mM amiloride hydrochloride significantly suppressed chorda tympani responses to a range of NaCl concentrations in C57BL/6J mice but did not do so consistently in 129/J mice. Amiloride failed to significantly suppress responses to a range of KCl concentrations in both mouse strains. The results suggest that for 129/J mice, sodium reception and transduction are primarily amiloride insensitive, whereas for C57BL/6J mice, both amiloride-sensitive and amiloride-insensitive components are present. The strain difference in NaCl intake may be mediated, in part, through gustatory mechanisms, with reduced preference for NaCl influenced by amiloride-sensitive sodium transduction mechanisms.
129/J和C57BL/6J近交系小鼠接受了48小时的两瓶偏好测试，比较氯化钠溶液和蒸馏水。129/J小鼠对0.08M氯化钠的偏好高于C57BL/6J小鼠。为了确定这种品系差异是否由味觉介导，研究了鼓索神经对一系列氯化钠和氯化钾浓度的综合神经反应。129/J和C57BL/6J小鼠对氯化钠的味觉神经反应相似。然而，在C57BL/6J小鼠中，舌部应用0.5mM盐酸阿米洛利可显著抑制鼓索神经对一系列氯化钠浓度的反应，但在129/J小鼠中并非始终如此。阿米洛利未能显著抑制两种小鼠品系对一系列氯化钾浓度的反应。结果表明，对于129/J小鼠，钠的接收和转导主要对阿米洛利不敏感，而对于C57BL/6J小鼠，存在对阿米洛利敏感和不敏感的成分。氯化钠摄入量的品系差异可能部分通过味觉机制介导，对氯化钠的偏好降低受阿米洛利敏感的钠转导机制影响。
