Bischoff R, Kolbe H V
Transgène S.A., Strasbourg, France.
J Chromatogr B Biomed Appl. 1994 Dec 9;662(2):261-78. doi: 10.1016/0378-4347(94)00203-7.
Non-enzymatic deamidation of asparagine and glutamine residues in proteins and peptides are reviewed by first outlining the well-described reaction mechanism involving cyclic imide intermediates, followed by a discussion of structural features which influence the reaction rate. The second and major part describes analytical techniques that allow studying deamidation in proteins using recombinant human growth hormone and recombinant hirudin as examples. Finally, the significance of non-enzymatic deamidation with respect to the production of pharmaceutical proteins is discussed.
本文首先概述了涉及环状酰亚胺中间体的蛋白质和肽中天冬酰胺和谷氨酰胺残基的非酶促脱酰胺反应机制，接着讨论了影响反应速率的结构特征，以此对蛋白质和肽中的非酶促脱酰胺反应进行综述。第二部分也是主要部分，以重组人生长激素和重组水蛭素为例，描述了用于研究蛋白质脱酰胺反应的分析技术。最后，讨论了非酶促脱酰胺反应在药用蛋白质生产方面的意义。
