Ferrero C A, Carobene A, Ceriotti F, Modenese A, Arcelloni C
Istituto Scientifico H. S. Raffaele, Laboratorio Analisi, Milano, Italy.
Clin Chem. 1995 Apr;41(4):575-80.
To further improve analytical accuracy in clinical chemistry, proficiency testing needs amelioration in the quality of materials tested and in target value assignment. To obtain information on the actual state-of-the-art in the Lombardy region of Italy, and to examine the behavior of different types of control materials (fresh-frozen human sera and lyophilized materials), we developed the following experimental design. Two human serum pools and two lyophilized sera were distributed to 32 laboratories for determination of glucose, creatinine, cholesterol, sodium, potassium, and gamma-glutamyltransferase (gamma-GT). Each analyte was measured in triplicate on each of 3 days. Target values for the controls were obtained with Reference Methods. The results show a good intralaboratory precision for every component but some accuracy problems for glucose, creatinine, cholesterol, and gamma-GT. Lyophilized materials showed some commutability problems for glucose, electrolytes, and gamma-GT, mainly with dry chemistry technology.
为进一步提高临床化学分析的准确性，能力验证需要在测试材料的质量和靶值设定方面加以改进。为了解意大利伦巴第地区的实际技术水平，并考察不同类型的质控材料（新鲜冷冻人血清和冻干材料）的性能，我们设计了如下实验。将两个人血清混合样本和两份冻干血清分发给32个实验室，用于测定葡萄糖、肌酐、胆固醇、钠、钾和γ-谷氨酰转移酶（γ-GT）。每种分析物在3天中的每一天都重复测定3次。对照的靶值通过参考方法获得。结果显示，每个组分的实验室内精密度良好，但葡萄糖、肌酐、胆固醇和γ-GT存在一些准确性问题。冻干材料在葡萄糖、电解质和γ-GT方面存在一些互换性问题，主要是在干化学技术方面。
