Graziani M S, Ceriotti F, Carobene A, Modenese A, Ferrero C, De Giorgi E, Franzin M, Marchetti C, Brambilla P, Bertona M, Giannone D, Righetti G, Marocchi A, Prencipe L, Franzini C, Luraschi P, Mocarelli P
Laboratorio di Chimica Clinica ed Ematologia, Ospedale Civile Maggiore, Verona, Italy.
Eur J Clin Chem Clin Biochem. 1997 Apr;35(4):311-5.
We report the results of an external quality assessment scheme for serum total cholesterol measurement involving about 100 Italian laboratories participating in an epidemiological study of post myocardial infarction. Two frozen human serum pools with Abell-Kendall assigned values are distributed quarterly at the laboratories (up to now seven events occurred); the obtained results are evaluated and discussed. In one exercise (# 5) duplicated measurements were repeated on three different days. Eighty-five to 98% of the laboratories obtained results within the total error limits (+/- 8.9%). But, while precision (calculated on the six replicates of exercise # 5) is good (90% of the laboratories obtained CV < 3%), inaccuracy problems are evident in every event. Indeed the mean bias from the reference method value ranged from 1.54 and 3.49% in the various events.
我们报告了一项针对血清总胆固醇测量的外部质量评估计划的结果，该计划涉及约100家参与心肌梗死后流行病学研究的意大利实验室。每季度向各实验室分发两个具有阿贝尔 - 肯德尔指定值的冷冻人血清样本池（截至目前已进行了七次）；对获得的结果进行评估和讨论。在一次检测（#5）中，在三个不同日期重复进行了重复测量。85%至98%的实验室获得的结果在总误差范围内（±8.9%）。但是，虽然精密度（根据检测#5的六次重复计算）良好（90%的实验室CV<3%），但每次检测中不准确问题都很明显。事实上，在不同检测中，与参考方法值的平均偏差范围为1.54%至3.49%。
