酪氨酸连接的真细菌S层糖蛋白的两种不同聚糖中“核心”结构的相似性。
Similarity of "core" structures in two different glycans of tyrosine-linked eubacterial S-layer glycoproteins.
作者信息
Messner P, Christian R, Neuninger C, Schulz G
机构信息
Zentrum für Ultrastrukturforschung, Universität für Bodenkultur, Vienna, Austria.
出版信息
J Bacteriol. 1995 Apr;177(8):2188-93. doi: 10.1128/jb.177.8.2188-2193.1995.
Previously, the repeating-unit structure of the S-layer glycoprotein from the eubacterium Bacillus alvei CCM 2051 has been determined to be [-->3)-beta-D-Galp-(1-->4)-[alpha-D-Glcp-(1-->6)-]-beta-D-ManpNAc- (1-->]n (E. Altman, J.-R. Brisson, P. Messner, and U. B. Sleytr, Biochem. Cell Biol. 69:72-78, 1991). Nuclear magnetic resonance spectroscopic reexamination of this glycan reveals that the O-antigen-like domain of the polysaccharide is [see text] connected with the S-layer polypeptide through the "core" structure -->3)-alpha-L-Rhap-(1-->3)-alpha-L-Rhap-(1-->3)-alpha-L-R hap-(1-->3)-beta-D-Galp-(1-->O)-Tyr. Except for the substitution in position 4 of the nonreducing rhamnose with the modified glyceric acid phosphate residue GroA-2-->OPO2-->4-beta-D-ManpNAc-(1-->, this core is identical to the core of the tyrosine-linked glycan from the S-layer glycoprotein of Thermoanaerobacter thermohydrosulfuricus L111-69 (K. Bock, J. Schuster-Kolbe, E. Altman, G. Allmaier, B. Stahl, R. Christian, U. B. Sleytr, and P. Messner, J. Biol. Chem. 269:7137-7144, 1994).
此前，已确定真细菌蜂房芽孢杆菌CCM 2051的S层糖蛋白的重复单元结构为[-->3)-β-D-吡喃半乳糖-(1-->4)-[α-D-吡喃葡萄糖-(1-->6)-]-β-D-甘露糖胺-(1-->]n（E. 阿尔特曼、J.-R. 布里松、P. 梅斯纳和U. B. 斯莱特，《生物化学与细胞生物学》69:72 - 78，1991年）。对该聚糖进行核磁共振光谱重新检查发现，多糖的O抗原样结构域通过“核心”结构-->3)-α-L-鼠李糖-(1-->3)-α-L-鼠李糖-(1-->3)-α-L-鼠李糖-(1-->3)-β-D-吡喃半乳糖-(1-->O)-酪氨酸与S层多肽相连。除了非还原鼠李糖4位被修饰的甘油酸磷酸残基GroA-2-->OPO2-->4-β-D-甘露糖胺-(1--)取代外，该核心与嗜热栖热菌L111 - 69的S层糖蛋白酪氨酸连接聚糖的核心相同（K. 博克、J. 舒斯特 - 科尔贝、E. 阿尔特曼、G. 阿尔迈尔、B. 施塔尔、R. 克里斯蒂安、U. B. 斯莱特和P. 梅斯纳，《生物化学杂志》269:7137 - 7144，1994年）。
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