Nishimori I, Bratanova T, Toshkov I, Caffrey T, Mogaki M, Shibata Y, Hollingsworth M A
Eppley Cancer Institute, University of Nebraska Medical Center, Omaha 68198, USA.
J Immunol. 1995 May 1;154(9):4865-73.
Experimental autoimmune sialoadenitis was induced in PL/J (H-2u) mice by intradermal immunization with human carbonic anhydrase II (CAII) and adjuvant containing monophosphoryl lipid A and trehalose diorynomycolate. Mice immunized with CAII showed a significant increase in the number and size of foci with lymphocytic infiltration in the salivary gland compared with mice immunized with adjuvant alone and untreated mice. In mice immunized with CAII, lymphocytic foci were observed around intercalated and intralobular ducts in the salivary glands, resulting in atrophy and replacement of acinar units. The epithelial cells of salivary ducts adjacent to the lymphocytic foci showed both degenerative and regenerative changes. Similar lymphocytic infiltrations were observed in the pancreas and kidney of a few mice immunized with CAII. Among several mouse strains with different H-2 haplotypes (p, q, r, s, and u), strains bearing H-2s and H-2u were susceptible to CAII-induced sialoadenitis. These results indicate that sialoadenitis induced by the immunization of CAII in mice may serve as a disease model of Sjögren's syndrome and that CAII or its derived peptides in association with the MHC may be one Ag recognized by an autoimmune response in this syndrome.
通过用人碳酸酐酶II（CAII）以及含有单磷酰脂质A和海藻糖二分枝菌酸的佐剂进行皮内免疫，在PL/J（H-2u）小鼠中诱导出实验性自身免疫性涎腺炎。与仅用佐剂免疫的小鼠和未处理的小鼠相比，用CAII免疫的小鼠唾液腺中淋巴细胞浸润灶的数量和大小显著增加。在用CAII免疫的小鼠中，在唾液腺的闰管和小叶内导管周围观察到淋巴细胞灶，导致腺泡单位萎缩和被替代。与淋巴细胞灶相邻的唾液腺导管上皮细胞呈现出退行性和再生性变化。在用CAII免疫的少数小鼠的胰腺和肾脏中也观察到类似的淋巴细胞浸润。在几种具有不同H-2单倍型（p、q、r、s和u）的小鼠品系中，携带H-2s和H-2u的品系对CAII诱导的涎腺炎易感。这些结果表明，在小鼠中通过CAII免疫诱导的涎腺炎可能作为干燥综合征的疾病模型，并且CAII或其衍生肽与主要组织相容性复合体相关可能是该综合征中自身免疫反应识别的一种抗原。
