Yang H, Koyanagi M, Matsumoto I, Shimada T, Han L, Aikawa T
Department of Physiology, School of Medicine Nagasaki University, Japan.
Life Sci. 1995;56(20):1727-39. doi: 10.1016/0024-3205(95)98580-9.
Adrenocortical secretory responses to chemical mediators and ACTH in CD1 ICR nu/nu (athymic) mice were compared with those in CD1 ICR (normal) mice. The bilateral adrenals of normal or athymic mice were perfused in situ with artificial medium equilibrated by 95% O2 + 5% CO2. Infusion of serotonin induced the secretory response of corticosterone significantly at 10 nM and markedly at 100 nM and the response at 1000 or 10000 nM declined as compared with that at 100nM in normal mice. Total corticosterone secretion in response to 100 or 1000 nM serotonin in athymic mice was about one fourth that in normal mice, respectively. Corticosterone responses to ACTH at the range of 10 to 300 pg/ml in athymic mice were comparable to those in normal mice. Infusion of histamine, platelet activating factor(PAF), or compound 48/80 did not induce significant corticosterone response in both normal and athymic mice. The data suggest that the congenital defect of the thymus and/or hair causes the hyporesponsiveness of adrenocortical cells to serotonin although the adrenal cortex of athymic mice is able to perform its function in response to ACTH.
将CD1 ICR nu/nu（无胸腺）小鼠和CD1 ICR（正常）小鼠对化学介质和促肾上腺皮质激素（ACTH）的肾上腺皮质分泌反应进行了比较。用95% O2 + 5% CO2平衡的人工培养基对正常或无胸腺小鼠的双侧肾上腺进行原位灌注。输注血清素在10 nM时显著诱导皮质酮分泌反应，在100 nM时明显诱导，与正常小鼠中100 nM时相比，1000或10000 nM时的反应下降。无胸腺小鼠对100或1000 nM血清素的总皮质酮分泌分别约为正常小鼠的四分之一。无胸腺小鼠在10至300 pg/ml范围内对ACTH的皮质酮反应与正常小鼠相当。输注组胺、血小板活化因子（PAF）或化合物48/80在正常和无胸腺小鼠中均未诱导出显著的皮质酮反应。数据表明，胸腺和/或毛发的先天性缺陷导致肾上腺皮质细胞对血清素反应低下，尽管无胸腺小鼠的肾上腺皮质能够对ACTH发挥其功能。
